摘要
目的:探讨银杏叶提取物(EGb)对体外培养的大鼠大脑皮质星形胶质细胞(Ast)增殖周期的影响。方法:体外培养、纯化及鉴定大鼠大脑皮质Ast;MTT比色法选择EGb作用浓度;1×10-7μL·L-1EGb50μL分别加入对照组和实验组培养液;利用流式细胞仪分析技术检测细胞周期。结果:随着Ast培养时间的延长,12h内EGb诱导Ast进入S期+G2/M期的细胞百分率有上升趋势,但24h后稍有下降。结论:EGb可通过影响Ast的细胞周期促进Ast的增殖。
Aim：To explore the effects of Ginkgo biloba extract（EGb） on cell increasing cycle in cultured astrocytes from cerebral cortex of rat in vitro. Methods：Astrocytes from rat cerebral cortex were cultured,purified and identified. The concentration of the drugs were selected by MTT assay. 50 μL EGb（1×10-7 μL·L-1） were added to the control group and experimental group respectively. Cell cycle were tested by flow cytometry. Results：During culturing with EGb,the percentage of the astrocytes in cell S＋G2/M phase was increased within 12 hours,but decreased a little after 24 hours. Conclusion：There is direct effect on cell cycle of astrocytes in rats treated with EGb.
出处
《中国临床神经科学》
2011年第1期60-64,共5页
Chinese Journal of Clinical Neurosciences
基金
山东省中医局课题(编号:2007-164)
泰安市科技局课题(编号:2008-44)
