摘要
目的:建立高效液相色谱法测定六味地黄大蜜丸中糖转化产物5-羟甲基糠醛的含量。方法:采用AgilentTCC18色谱柱,甲醇-0.1%醋酸(3∶97)为流动相,柱温:30℃,流速:1.0mL·min-1;检测波长:284nm。结果:5-羟甲基糠醛进样量在0.00054～2.2μg范围内线性关系良好(r=1.000),最低检出限为2.1581×10-4μg,加样回收率(n=6)为100.1%(RSD%=1.7%)。结论:本法简便、准确,可用于六味地黄大蜜丸中5-羟甲基糠醛的含量测定。
Objective：To establish an HPLC method for determination of 5-hydroxymethyl furfural（5-HMF）.Method：RP-HPLC was adopted.The separation was performed on Agilent TC C18（250 mm×4.6 mm,5 μm）colum with the mobile phase of methanol-0.1%aqueous acetic acid（3∶97）at 25 ℃.The detection wavelength was set at 284 nm.Results：The linear ranges were 0.00054-2.2 μg,the lowest detection limit was 2.1581×10-4 μg,The average recovery（n=6）was 100.1%（RSD%=1.7%）.Conclusion：A rapid,sensitive and accurate method can provide a scientific basis for the quality control of Liuwei Dihuang（big honeyed pills）.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2010年第12期2349-2351,共3页
Chinese Journal of Pharmaceutical Analysis
关键词
六味地黄大蜜丸
5-羟甲基糠醛
Liuwei Dihuang（big honeyed pills）
5-hydroxymethyl furfural
