摘要
目的:研究人类疱疹病毒8型(HHV-8)K13(v-FLIP)基因编码蛋白分别在人血管内皮细胞和前列腺癌细胞中的表达及其对上述两种细胞增殖能力的影响。方法:将PEF-K13-Flag-IRES/Puro重组质粒瞬时转染人血管内皮细胞系EA.HY926细胞和前列腺癌细胞系PC-3细胞,采用Western blot方法检测K13基因编码蛋白表达情况;分别运用流式细胞术和MTT方法检测K13基因编码蛋白对这两种细胞增殖的影响。结果:HHV-8 K13基因编码蛋白能够在人血管内皮细胞和前列腺癌细胞中表达,流式细胞分析和MTT结果显示,HHV-8 K13基因编码蛋白对上述两种细胞的增殖均有明显的抑制作用(P<0.05)。结论:HHV-8 K13基因编码蛋白可以明显抑制人血管内皮细胞和前列腺癌细胞的增殖。
Objective：To investigate the expression protein of HHV-8 K13 in human endothelial and prostate cancer cells respec-tively,and evaluate the effect on the proliferation of the two cell lines.Methods：The recombinant plasmid PEF-K13-Flag-IRES / Puro was transiently trancfected into EA.HY926 cells and PC-3 cells.The expression of K13 protein in both cells was analyzed by Western blot.The function of K13 protein in cell cycle and cell proliferation were measured by flow cytometry and MTT assay,respectively.Results：The specific band of K13 protein was detected at the expected place by Western blot.In addition,the results of the flow cy-tometry and MTT assay showed that the HHV-8 K13 protein could significantly inhibit the proliferation in human endothelial and prostate cancer cells.Conclusion：HHV-8 K13 protein might inhibit the growth of human endothelial and prostate cancer cells.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2011年第1期21-25,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
中国博士后基金资助(20080441064)
