摘要
目的:研究金丝桃苷(hyperfine,HP)对CCl4诱导的大鼠肝损伤的保护作用。方法:连续3d对大鼠灌胃给予不同剂量的HP,并于末次给药6h后灌胃给予CCl4复制急性肝损伤模型,24h测定血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)及肝组织匀浆中丙二醛(MDA)含量和超氧化物歧化酶(SOD)、谷胱甘肽(GSH)活性;同时进行肝组织病理学观察。结果:不同剂量的HP可显著降低模型大鼠血清中ALT、AST,肝组织匀浆中MDA含量;显著升高SOD、GSH活性;病理切片表明,HP预处理组肝组织损伤均有不同程度减轻,其中以30mg·kg-1剂量效果最佳。结论:HP对CCl4诱导的大鼠急性肝损伤具有较好的保护作用,其作用机制可能与HP抑制抗氧化酶活性和抗自由基活性有关。
OBJECTIVE： To study the protective effect of hyperfine （HP） on the acute liver injury in rats induced by CCl4. METHODS ： Different dosage of HP was given to rats intragastrically for 3 days. Acute liver injury was induced by CCl4 with intragastric administration 6 h after last administration. After 24 hours, the content of ALT and AST in the serum, MDA content, the activity of SOD and GSH in the liver homogenate were determined. At the same time, the pathological observation of liver tissues was conducted. RESULTS： The different dosages of HP could decrease the content of AST and ALT in serum and MDA content in the liver homogenate significantly. And the activity of SOD and GSH increased significantly. The pathological sections showed that liver injury was relieved to different extent in HP pre-treatment group. CONCLUSION： HP has good protective effect on the acute liver injury in rats induced by CCl4. Its mechanism might be related with the inhibition effect of HP on antioxidant enzyme activities and anti-radicals activity.
出处
《中国药房》
CAS
CSCD
北大核心
2011年第7期582-583,共2页
China Pharmacy
基金
国家自然科学基金资助项目(30572366)
