摘要
探讨高产胞外多糖嗜热链球菌混菌培养的产酸、产香、产黏特性。以发酵酸度、乙醛质量浓度和黏度为综合指标,确定高产胞外多糖的嗜热链球菌(S.t.)、保加利亚乳杆菌(L.b.)、嗜酸乳杆菌(L.a.)混菌最佳培养条件为培养温度42℃,总接菌量体积分数4%,V(S.t.):V(L.b.):V(L.a.)=3:2:1。在此条件下,混菌培养可以显著地提高酸度、乙醛质量浓度、黏度、胞外多糖质量浓度、β-半乳糖苷酶酶活、蛋白酶水解能力(P<0.01)。发酵过程中,胞外多糖质量浓度与黏度,β-半乳糖苷酶酶活与酸度,蛋白酶酶活与酸度、乙醛质量浓度的相关性系数很高。
The aim of this research is to study the acidity,acetaldehyde and viscosity of mixed cultivation with high exopolysaccharides-producing Streptococcus thermophilus.The optimal fermentation conditions with mixed cultivation are：42 ℃,inoculation size 4%（v/v）,the strains matching ratio of high exopolysaccharides-producing Streptococcus thermophilus,Lactobacillus bulgaricus and Lactobacillus acidophilus is 3：2：1 according to acidity,content of acetaldehyde and viscosity.On this condition,mixed cultivation of Lactic acid bacteria significantly improve acidity,acetaldehyde content,viscosity,exopolysaccharide（EPS）,the activity of β-galactosidase and protease（P0.01）.The correlative modulus of EPS and viscosity,the activity of β-galactosidase and acidity,the activity of protease and acidity,acetaldehyde content is high.
出处
《中国乳品工业》
CAS
北大核心
2011年第1期15-18,共4页
China Dairy Industry
基金
辽宁省教育厅创新团队项目(2009T013)
关键词
胞外多糖
混菌培养
酸度
黏度
Exopolysaccharide（EPS）
mixed cultivation
acidity
viscosity
