摘要
目的为减少、避免输血的不良反应,确保临床用血安全。方法对12例不规则抗体阳性标本用微柱法和聚凝胺法做抗体筛查进行比较,用谱细胞进行特异性检测。结果 12例受血者中抗体筛查微柱法和聚凝胺法均为阳性,阳性率为100%,两法比较无差异;抗体效价检查微柱法比聚凝胺法灵敏度高(P<0.05),Rh系统阳性率为41.6%,其他系统阳性率为8.3%。结论两法对不规则抗体阳性检测的符合率及谱细胞检测阳性率均无差异,但对抗体的灵敏度微柱法比聚凝胺法灵敏度高,并且微柱法操作标准化、自动化、重复性好,所需标本量少,结果易判读,应作为抗体筛查的首选方法。
Objective To reduce and（or） avoid the adverse reaction of transfusion and ensure the security of transfusion. Methods Irregular antibody screenings with polybrene-method were compared with the ones with microplate-method. For those whose antibody testing were positive, the specific testing was performed by panel cell method. Results The antibody screening for the twelve blood receivers demonstrated all positive both in polybrene and microplate method, the coincidence rate was 100%. Conclusion There were no statistically significant differences between these two methods on the test of the irregular antibodies, however, the sensitivity to the antibody of the microplate-method was higher than the one of the polybrene-method, meanwhile the microplate-method was not only easy to be performed standarizationally, automatically and repeatetionally, but also was required small sample and was easy to read, therefore, microplate-method was recommended to be the first choice tool for the antibody screening.
出处
《中国临床新医学》
2010年第12期1213-1214,共2页
CHINESE JOURNAL OF NEW CLINICAL MEDICINE
关键词
抗体
输血
微柱法
聚凝胺法
Antibody
Transfusion
Microplate-method
Polybrene-method
