摘要
目的:建立柴芩清肝方提取物中柴胡皂苷a的含量测定方法。方法:采用Diamonsil C18柱(4.6 mm×200 mm,5μm),以乙腈-水为流动相进行梯度洗脱,流速1.0 mL·min-1,蒸发光散射检测器进行检测,漂移管温度105℃,氮气流速2.0L·min-1。结果:柴胡皂苷a进样量在1.7～8.5μg与峰面积对数线性关系良好(r=0.999 4),平均回收率为96.6%,RSD1.8%(n=5)。结论:方法专属性强、准确、快速,适用于柴芩清肝方提取物中柴胡皂苷a的含量测定。
Objective：To establish a HPLC method for the determination of saikosaponin A in the extract of Chaiqin Qinggan formula.Method：The column of Diamonsil C18（4.6 mm×200 mm,5 μm） was used.The mobile phase was acetonitrile-water with a gradient elution program with the flow rate at 1.0 mL·min-1.The evaporative light scattering detector（ELSD） was used as detector.Drift tube temperature was at 105℃,and the gas flow rate was 2.0 L·min-1.Result：The logarithmic calibration curves were linear in the range of 1.7-8.5 μg（r=0.999 4）.The average recovery was 96.6% with the RSD value of 1.8%（n = 5）.Conclusion：The method can be used to determine the content of saikosaponin a in the extract of Chaiqin Qinggan formula.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第2期57-59,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
黑龙江省科技攻关项目资助(GC08C322)
