摘要
目的:了解小儿急性呼吸道感染(acute respiratory tract infection ARTI)中病毒感染状况,为预防和治疗疾病提供依据。方法:收集2009年5月～2010年4月期间340例确诊为ARTI住院患儿的深部鼻咽分泌物(NPS)临床标本,采用逆转录聚合酶链反应(RT-PCR)法检测常见的7种呼吸道病毒:呼吸道合胞病毒(RSV)、流感病毒(IFV)、副流感病毒(PIV)、人类偏肺病毒(HMPV)、腺病毒(ADV)、鼻病毒(HRV)和肠道病毒(EV)。统计分析各种病毒所致ARTI的临床特点及流行特征。结果:340例标本中共检出212例阳性,阳性率为62.35%。其中RSV感染为132例,阳性率为62.3%;IFV感染为45例、阳性率为21.3%;PIV感染为13例,阳性率为6.1%;HMPV感染为13例,阳性率为6.1%;ADV感染为4例,阳性率为1.9%;HRV感染为2例,阳性率为0.9%;EV感染为7例,阳性率为3.3%。结论:本地区诊断为ARTI住院儿童中病毒感染率为62.35%,其中以RSV为主。
Objective： To detect the presence of common viral pathogens in clinical specimens of respiratory tractand to understand the current situation of viral infection, children’s acute respiratory tract infection（ARTI） in Nanjing Children’s Hospital. Methods： Deep nasopharyngeal secretions（NPS） specimens were collected in Hospitalized children diagnosed with ARTI. A variety of viral pathogens including respiratory syncytial virus（RSV）, influenza virus（IFV）, parainfluenza virus（PIV）, human metapneumovirus （HMPV）, adenovirus （ADV）, rhinovirus （HRV） and enterovirus（EV） were detected using reverse transcription polymerase chain reaction（RT-PCR）. Meanwhile, the clinical and epidemiological manifestations of ARTI caused by these viral pathogens were analyzed. Results：A total of 212 cases affected with viral pathogens were detected in 340 specimens （62.35%）. Among them, 132 cases were RSV infection （62.3%）, 45 cases IFV infection （21.3%）, 13 cases PIV infection （6.1%）, 13 cases HMPV infection （6.1%）, 4 cases ADV infection （1.9%）, 2 cases HRV Infection （0.9%）, and 7 cases EV infection （3.3%）. Conclusion：RSV infection is most common in ARTI children. Few children have multiple viral infections.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第12期1756-1759,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家863子课题资助(2002AAZ204153)
