摘要
目的观察脂联素对胰岛β细胞胰岛素分泌、UCP-2、PPARγmRNA表达和凋亡的影响。方法大鼠胰岛细胞株RIN-m5F分别用5.6mmol/L和16.7 mmol/L葡萄糖,并加入不同浓度的球形脂联素(0～2500μg/L)培养48 h。放免法测定胰岛素含量,RT-PCR检测UCP-2、PPARγmRNA的表达。流式细胞仪检测细胞凋亡率,Western Blot检测激活型Caspase3的表达。结果高糖培养下脂联素同时干预后基础胰岛素分泌显著降低,高糖刺激的胰岛素分泌显著增加。和高糖组比较,脂联素组UCP-2、PPARγmRNA的表达显著降低(P<0.05),细胞凋亡率和激活型Caspase-3的表达显著降低(P<0.05)。结论脂联素能改善高糖所致的β细胞胰岛素分泌异常,其机制可能与通过下调PPARγ降低UCP-2的表达有关。脂联素可拮抗高糖所致的β细胞凋亡。
Objective To observe the effects of globular adiponectin on insulin secretion function, mRNA expression of UCP 2 and PPARγand apoptosis in pancreatic - cells. Methods Rat insulinoma cell line RIN-mSF was cultured in 5.6 mmol / L glucose and 16.7retool / L glucose respectively, with or without different concentration of rat globular adiponectin intervention（0 - 2500μg/l）for 48 hours. Basal and high glucose- stimulated insulin secretion （GSIS）in the medium was measured by radioimmunoassay （RIA）. UCP-2 and PPARγ mRNA expression in β cells was detected by RT PCR. Ceil apoptosis was detected by flow cytometric analysis and the protein expression leveI of cleaved easpase 3 was detected by Western blot. Results Basal insulin secretion significantly decreased but GSIS significantly increased after adiponectin intervention under high glucose condition. Compared with control group, UCP-2 and PPAR7 mRNA expression significantly increased in high glucose group （P〈 0.05）, but it decreased in group with adiponectin intervention（P〈0.05）. After co-incubation with adiponectin, apoptotic cells and the protein expression level of cleaved caspase 3 were significantly decreased compared with that of the high glucose group（P〈0.05）. Conclusions Adiponectin can restore the secretion fuction of 13 cells impaired by chronic exposure to high glucose level, which may be related to the mechanism of reduced UCP-2 mRNA expression regulated by adiponectin through PPAR7 pathway. Adiponectin can counteract high glucose -induced apoptosis of the pancreatic beta cells by inhibiting caspase-3 activation.
出处
《中国糖尿病杂志》
CAS
CSCD
北大核心
2010年第12期930-933,共4页
Chinese Journal of Diabetes
基金
国家自然科学基金资助项目(30570756)
