摘要
激光扫描共聚焦显微镜（laser scanning confocal microscope,LSCM）是20世纪80年代发展的高科技产品,主要是采用激光作为光源,在荧光显微镜的基础上添加了激光扫描装置和计算机图像处理系统等,通过对观察样品进行光学断层扫描及三维结构重建等,可得到细胞或组织内部细微结构的荧光图像,同时激光扫描共聚焦显微镜还是活细胞动态观察、多重免疫荧光标记和Ca2＋荧光观察（Chen et al.,2009）的有力工具,目前已在花粉（Fang et al.,2008）和花粉管发育（Chen et al.,2008;Wu et al.,2008）、蛋白质功能（Zheng et al.,2009）及信号传导（Liu et al.。
The cytol development of male gametophyte of Bambusa multiplex was studied by dying with a fluorescence dye of Hoechst 33342 and observing with laser scanning confocal microscope（LSCM）.The result showed twice mitoses took place and the mature pollens had three cells with one aperture during the development of the male gametophytes,and the male gametophytes abnormally acted at a frequency of 38% in B.multiplex.With the confocal arrangement,serial optical scanning,and advanced three-dimensional reconstruction of the LSCM,direct and visual display of the three-dimensional pollen architecture was available in this study.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2010年第11期158-161,F0003,共5页
Scientia Silvae Sinicae
基金
国家自然科学基金项目(30771753)
"十一五"科技支撑项目(2006BAD19B0202)
浙江省科技厅优先主题重点农业项目(2010C12011)
浙江林学院启动基金(2451001131)
关键词
孝顺竹
花粉粒发育
激光扫描共聚焦显微镜
Bambusa multiplex
development of pollen
laser scanning confocal microscope
