摘要
目的：研究ＶＥＧＦ，ｐ５３和ｐ２１ｒａｓ在原发性肝细胞癌（ＨＣＣ）的表达和三者间的相关性及其与ＨＣＣ病理分级，转移行为的关系。方法：应用免疫组织化学Ｓ－Ｐ法对３４例ＨＣＣ的癌组织中的ＶＥＧＦ，ｐ２１ｒａｓ和ｐ５３的表达作了研究。结果：ＶＥＧＦ，ｐ２１ｒａｓ和ｐ５３三者在肝癌和癌旁组织的阳性率均有非常显著的差异（Ｐ＜０．０１），ＶＥＧＦ的表达与ｐ２１ｒａｓ和ｐ５３均呈显著的正相关（Ｐ＜０．０１）。三者间的协同表达亦与ＨＣＣ的病理分级和转移行为有关，而与肿瘤大小，ＡＦＰ水平，ＨＢｓＡｇ阳性无关。结论：ＶＥＧＦ可能在ＨＣＣ的形成，发展和转移中与ｐ５３和ｒａｓ基因协同作用，三者的协同作用可能与ＨＣＣ的病理分级和转移行为有关。
Objective:To study the correlation between expressions of
VEGF,p53,p21ras and cell differentiation and metastasis in hepatocellular
carcinoma(HCC).Methods:34 cases of HCC were selected for this study.Expressions of
VEGF,Mutant p53(M53)and p21ras pro-tein were synchronously detected and compared by
immunohistochemical S-P method.Results:The positive rates of VEGF,Mp53,p21ras in HCC
were very significantly higher than that of PT(<0.01).VEGF and Mp53 protein were positive
simultaneously in 17(50%)in HCC which had positive correlation(P<0.01);VEGF and p21ras
protein were positive simulaneously in 22(64.7%)which had positive correlation too(P<0.01);A
signifficant correlation was found between the coexpression was associated with the degree of
histologic differentiation and metastasis behavior in HCC,but not with tumor size,AFP levels and
HBsAg infection.Conclusion:The results showed the expression of VEGF was related with p53
and p21ras.The coexpression of them may be useful marks for evaluating the malignant
degree,the metastasis and invasive ability of HCC.
出处
《中国现代医学杂志》
CAS
CSCD
1999年第4期6-8,共3页
China Journal of Modern Medicine
关键词
肝细胞癌
血管内皮
生长因子
P53基因
RAS基因
Hepatocellular
carcinoma
Vascular endothelial growth factor
p53 gene
p21 gene
Immunohistochemistry
