摘要
采用4h51Cr释放法，分别以小鼠脾脏淋巴细胞对YAC-1细胞和肿瘤患者外周血淋巴细胞对K562细胞的体外自然杀伤（NK）活性为指标，研究受电离辐射后小鼠脾脏NK细胞活性的变化以及干扰素（IFN－α）对NK细胞活性的影响；并观察肿瘤患者及其放疗后NK细胞活性的变化以及IFN－α的调节作用。结果表明NK细胞具有较强的辐射耐受性，经16Gy照射后24hNK细胞活性显著降低；肿瘤患者的NK细胞活性明显低于正常对照组（P＜0．01），放疗后NK细胞活性进一步降低（P＜0．05）。IFN－α处理NK细胞可以显著增强NK细胞活性，这提示IFN-α有可能作为一种生物应答调节剂用于肿瘤患者、肿瘤放、化疗或手术患者。
The effect of radiation on natural killer cell (NK) activity and the modulatory effect of interferon (IFN-α) were studied in vitro on mouse splenic lymphocytes against YAC-1 cells and peripheral lymphocytes of cancer patients against K562 cells, using 4hr 51Cr release method. The results showed that NK cells were relatively radioresistant. NK cell activity decreased significantly 24hours after 16 Gy 60Coγ-ray irradiation (P<0.01). NK cell activity of cancer patient was significntly lower than that of normal control (P<0.01) and was decreased further after radiotherapy (P<0.05).The NK cell activity was enhanced after radiotherapy by IFN-α treatment both in normal and irradiated mice, as well as in healthy subjects and cancer patients. The results suggested that IFN-α as a biological response modifier (BRM) would be valuable in the treatment of cancer patients, following operation and high-dose radiotherapy or chemotherapy.
出处
《基础医学与临床》
CSCD
1999年第3期53-56,共4页
Basic and Clinical Medicine
