摘要
为了明确通过RNA干扰技术(RNAi)获得的转基因小麦品系"Glu-1Dx5-RNAi"中1Dx5基因的遗传规律,以"Glu-1Dx5-RNAi"为供体亲本,以弱筋品种扬麦18和扬麦13为轮回亲本进行回交,采用半籽粒SDS-PAGE法检测亲本、F_1、F_2和BC_1F_1籽粒的高分子量谷蛋白亚基(HMW-GS)组成。结果表明,1Dx5基因的沉默效应在杂交后代中表现为显性遗传,在F_2和BC_1F_1世代1Dx5亚基不表达的种子数与1Dx5亚基表达的种子数的比例分别符合13:3和3:1的分离比例,说明转小RNA基因导致1Dx5基因沉默是单基因显性遗传。对转基因小麦"Glu-1Dx5-RNAi"及其受体品种Bobwhite进行品质测试表明,Glu-1Dx5-RNAi蛋白质含量(13.28%)比转化受体Bobwhite(12.83%)高0.45个百分点,硬度指数、微量SDS沉降值比受体品种分别降低3.13和3.7 mL。1Dx5基因沉默对弱筋小麦育种可能会有一定的利用价值。
High-molecular-weight glutenin subunit（HMW-GS） variation is related with the wheat processing quality.To investigate the genetic behavior of silent 1Dx5 gene in RNAi transgenic wheat, the transgenic wheat line Glu-lDx5-RNAi was used to hybridize with two soft wheat varieties Yang-mai 18 and Yangmai 13 which were adopted as recurrent parents in backcrosses.The HMW-GS distribution in parents,and their F_1,F_2 and BC_1F_1 progenies were analyzed by the SDS-PAGE technique. Results showed the silent effect of 1Dx5 gene is dominant and can stably inherit in transgenetic progenies. The segregation ratio of lines without 1Dx5 subunit and lines with 1Dx5 subunit is 13：3 and 3： 1 in F_2 and BC_1F_1 progeny,respectively.The silent 1Dx5 gene by RNAi may inherit as a single dominant genetic factor.Quality tests of transgenic wheat varieties and receptor Bobwhite showed that the total protein content of transgenic lines was 13.28%,which was slightly higher than that of the receptor lines with protein content of 12.83%.Moreover,the hardness index and SDS sedimentation value reduced by 3.13 and 3.7 mL in transgenic wheat varieties than that in receptor,respectively.
出处
《麦类作物学报》
CAS
CSCD
北大核心
2010年第6期991-996,共6页
Journal of Triticeae Crops
基金
南方麦区弱筋小麦转基因品质改良项目(2008ZX08002-004)
国家重点基础研究发展计划("973"计划)项目(2009CB118300)
小麦现代农业产业技术体系项目(nycytx-03)
