摘要
从制备的番茄花叶病毒（ＴｏＭＶ－ Ｓ１） 抗血清中提纯ＩｇＧ，并用过碘酸钠法进行辣根过氧化物酶（ＨＲＰ） 标记，建立了检测ＴｏＭＶ 的ＤＡＳ－ ＥＬＩＳＡ 系统。利用此检测系统并结合生物学方法，对浙江及山西等地的田间病样进行测定，结果发现茄子、番茄等茄科作物中均有ＴｏＭＶ 的侵染，其中茄子中有５０ ％ 左右的发生率，番茄中ＴｏＭＶ 发生率南北差异较大， 在浙江发生率仅为１０ ％ 左右，而在山西有６０ ．７ ％ 的发生率，辣椒中未测到ＴｏＭＶ。这是在我国首次进行ＴｏＭＶ
Immunoglobulin (IgG) of ToMV antiserum was purified and conjugated with horseradish peroxidase (HRP) by sodium periodate. A DAS-ELISA method for ToMV detection was developed using the conjugant. Some field samples of Solanaceae collected from Zhejiang and Shanxi Provinces were detected by DAS-ELISA and biological tests, results showed that ToMV was a common pathogen of tomato and eggplant. About 50% of detected Solanum melongena samples had ToMV both in Zhejiang and Shanxi provinces.However,there was a significant difference between tomato samples from Zhejiang and Shanxi provinces.In Zhejiang province, only about 10% of samples were infected by ToMV, while the numbers of infected samples were above 60% in Shanxi. No sample was found infected by ToMV in Capsicum annum . This is the first report about the field survey of ToMV in China.
出处
《植物病理学报》
CAS
CSCD
北大核心
1999年第2期157-162,共6页
Acta Phytopathologica Sinica
基金
浙江省自然科学基金
浙江省青年科技人才培养专项资金
教育部"跨世纪优秀人才培养计划"基金
