摘要
为探讨不同耐铝小麦品种铝胁迫下根尖细胞结构变化及活性氧代谢差异,选用耐铝型小麦品种Atlas 66和铝敏感型品种Scout 66进行了试验研究。结果表明:50mmol/L AlCl3处理24 h,Atlas 66和Scout 66根尖细胞伸长受抑制程度均随胁迫时间延长(6、12和24 h)而增强,根尖细胞相对长度与根相对伸长率呈显著正相关的关系(R2=0.919);Atlas 66和Scout 66根尖细胞均受到不同程度的破坏,具体表现为根尖伸长区皮层细胞变扁平,细胞间隙变小,Scout 66受破坏程度比Atlas 66显著,表明根尖细胞结构受损导致细胞伸长受阻,从而造成根伸长受抑制。50mmol/L AlCl3处理6 h,Atlas 66和Scout 66根尖超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、过氧化物酶(POD)和谷胱甘肽还原酶(GR)等酶活性升高,增幅分别为8.2%、82.0%、44.0%、25.0%、23.5%和23.3%、33.6%、26.9%、32.5%、14.8%,说明AlCl3处理引起活性氧代谢酶活性的升高,不同耐铝小麦品种抗氧化胁迫能力差异显著。
Al-tolerant wheat （Triticurnaestivum L. ） Atlas 66 and Al-sensitive wheat Scout 66 were studied to elucidate the differences on cell structure and ROS metabolism in root tip under A1 stress. The results showed that both Atlas 66 and Scout 66 showed a retardation of root cell elongation after treated with 50.0 μmol/L A1 for 6,12 and 24 h. RRE （relative root elongation） and RCL （relative cell length） was well correlated （R2 =0. 919）. Root tip cells of Atlas 66 and Scout 66 were damaged with different degree. Longitudinal sections revealed that the epidermal cells in the elongation zone became flat, intercellular space decreased after 24 h of 50. 0 μmol/L A1C13 treatment. Damages to cell structure on Scout 66 were more serious than that on Atlas 66. The results indicated that the inhibition of cell elongation was due to the damage of cell structure, thus inhibited the elongation of root. After the treatment of 50. 0 μmol/L A1C13 for 24 h, the activity of superoxide dismutases （SOD）, catalase （CAT）, ascorbate peroxi- dase （APX）,peroxidases （POD） and glutathionereductase （GR） in Atlas 66 and Scout 66 increased by 8. 2%,82. 0 %,44.0%,25.0%,23.5% and 23.3%,33.6%,26.9%,32.5%,14. 8%,respectively. The re- sults indicated that increased activities of enzymes in ROS metabolism were induced by the application of A1 and that difference in ROS metabolism can cause the Al-tolerant difference among different Al-tolerant wheat varieties.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2010年第6期715-720,共6页
Journal of Huazhong Agricultural University
基金
国家自然科学基金项目(30571115)资助
关键词
小麦
铝毒
细胞显微结构
活性氧代谢酶
wheat（Triticum aestivum L. ）
aluminum toxicity
cell microstructure
ROS metabolism enzymes
