摘要
通过对海南槟榔黄化病病原的研究,初步确定其病原物,对开展后续研究及防治策略提供依据。利用巢式PCR方法,对在海南3个市县采集的28个黄化病植株的不同组织进行了植原体检测,结果表明:在2个市县9个槟榔黄化病病株叶片中检测到了植原体,而在根和茎中没有发现植原体。将扩增片段克隆后测序得到1249bp的完整植原体序列。利用MEGA3软件构建了系统发育进化树,同源性聚类发现其属于翠菊黄花组B亚组。初步判断发生在海南的槟榔黄化病是由于植原体引起。
Yellow leaf disease （YLD） of the areca nut palm was a severe disease that occurred mainly in Southeast Asia.Although YLD has similar symptoms in different regions,it has been proven to be induced by different pathogens.In this work,28 samples of diseased palms were collected from three different counties of Hainan Province,China.Their 16S rDNA was studied by the nested PCR to detect the phytoplasma associated with YLD.The results showed that amplification was observed only in leaf samples,but not in stem or root samples,and phytoplasmas were detected only in 9 samples collected from 2 counties of Hainan pronvice.High boostrap values suggested that arecanut YLD phytoplasma is most closely related to Paulownia Witches ＇ Broom Phytoplasma and is a member of the Aster yellows group （group 16SrⅠ-B）.
出处
《中国农学通报》
CSCD
北大核心
2010年第22期381-384,共4页
Chinese Agricultural Science Bulletin
基金
"十一五"国家科技支撑计划课题"重点道地南药良种选育及规范化生产关键技术研究"(2007BAI27B03)
关键词
槟榔
黄化病
植原体
巢式PCR
Areca catechu L.
yellow leaf disease
phytoplasma
nested PCR
