摘要
背景:为了进一步拓展羟基磷灰石应用范围,提高其实用性,课题组制备了两种具有不同功能的超细羟基磷灰石生物复合材料:载银羟基磷灰石和壳聚糖表面改性羟基磷灰石。目的:观察两种新型羟基磷灰石生物复合材料的抑菌性和稳定性。方法:以氢氧化钙和磷酸二氢钙为前驱,用均相化学沉淀法合成制备了羟基磷灰石。以此为基础研究制备载银羟基磷灰石和壳聚糖表面改性羟基磷灰石,采用抑菌圈法定性检验载银羟基磷灰石的抑菌性,通过测定Zeta电位及平均粒度检验壳聚糖表面改性羟基磷灰石的稳定性。结果与结论:载银率为1.2%的羟基磷灰石对大肠杆菌和金黄色葡萄球菌均有较强的抑菌作用,其抑菌圈直径分别为10mm,11mm;羟基磷灰石微粒的表面电荷受pH值及壳聚糖加入量等因素的影响,pH值向大于6.8或小于6.8变化,壳聚糖加入量在一定范围内(≤0.12%),均能使Zeta电位绝对值越大,稳定性增强。用壳聚糖对普通的羟基磷灰石粒子进行改性,可得到荷正电且稳定存在的羟基磷灰石水悬浮液。
BACKGROUND：In order to expand the application scope and practicality of hydroxyapatite,two different functional ultra-fine hydroxyapatite composites were prepared：silver-carrying hydroxyapatite and chitosan surface modified hydroxyapatite.OBJECTIVE：To investigate antibacterial activity and stability of two new hydroxyapatite biocomposite.METHODS：Using calcium hydroxide and calcium phosphate as the precursor,the hydroxyapatite was synthesized using homogeneous chemical precipitation method.Then silver-carrying hydroxyapatite and chitosan surface modified hydroxyapatite were prepared,antimicrobial activity of silver-carrying hydroxyapatite was determined using the inhibition zone test,the stability of chitosan surface modified hydroxyapatite was assayed through measuring the Zeta potential and average particle size.RESULTS AND CONCLUSION：The hydroxyapatite at 1.2% silver loading had a strong antimicrobial effect on Escherichia coli and staphylococcus aureus,the inhibition zone diameters were 10 mm and 11 mm respectively;the surface charge of hydroxyapatite particles was influenced by pH value and the quantity of chitosan added,etc.The absolute value of Zeta potential would increase and the stability is enhanced at a range of pH value 6.8 or 6.8 and the quantity of chitosan added is certain （≤ 0.12%）.The positively charged and stable hydroxyapatite aqueous suspension can be obtained by chitosan modification of hydroxyapatite particles.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第38期7100-7102,共3页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
陕西省教育厅专项科研计划项目(06JK338)~~
