摘要
目的:建立高灵敏度测定样品中四环素的电位分析法。方法:基于分子印迹整体柱,以四环素-磷钨酸离子缔合物为电活性物质,构建成PVC为基质膜的高灵敏度四环素选择电极,并对电极性能指标进行评价。结果:在pH2～4溶液中,电极的线性范围为1×10-8～1×10-3mol.L-1,斜率为58.3mV/pC,检出限为约6×10-9mol.L-1,精密度和准确度好。结论:该电极灵敏度高,选择性好,测定结果准确可靠,可用于药片、人血清、水产品等样品中四环素的含量测定。
Objective：To establish a potentiometric method with high sensitivity to determine tetracycline in various samples.Method：A PVC membrane tetracycline selective electrode with high sensitivity was prepared with the ion-pair of tetracycline-phosphotungstic acid as the electroactive substance,based on molecularly imprinted monolithic column.And then the performance of the electrode was evaluated.Results：The electrode showed a linear Nernst response in the range of 1×10-8-1×10-3 mol· L-1 in a buffer solution of pH 2-4 with a slope of 58.3 mV/pC.The low limit of detection was about 6×10-9 mol· L-1,and the accuracy and precision were good.Conclusion：The proposed electrode is sensitive,selective,accurate and reliable,and thus then can be used for the determination of tetracycline in tablets,human serum and aquatic products,etc.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2010年第10期1904-1908,共5页
Chinese Journal of Pharmaceutical Analysis
基金
宁波市应用型专业人才培养基地项目(Jd090105)
宁波市科技局农业科研攻关项目(2007C10061)
浙江省教育厅自然科学基金(20070963)
浙江省科技厅新苗计划(2008R40G2070056)
宁波大学王宽诚幸福基金,宁波大学学科项目(xkl09071)资助
关键词
四环素
离子选择性电极
PVC膜
分子印迹整体柱
tetracycline
ion selective electrode
PVC membrane
molecular imprinted monolithic column
