摘要
目的观察阿米巴角膜炎患者角膜组织病理变化及相关细胞因子基质金属蛋白酶13(matrix metalloproteinase13,MMP-13)及成纤维细胞生长因子2(fibroblast growth factor2,FGF2)的表达。方法收集8例(8眼)阿米巴角膜炎患者接受角膜移植手术后切除的角膜组织,采用常规HE染色和抗MMP-13、抗FGF2免疫组织化学染色,观察其病理变化及MMP-13和FGF2的表达。结果病程在1个月之内,角膜基质坏死,可见中性粒细胞为主的炎性细胞浸润,组织内可见大量包囊及滋养体。同时,MMP-13呈强阳性表达,平均光密度值为0.3085±0.0781;FGF2表达较弱,平均光密度值为0.2057±0.0387;病程1个月至半年内,患者角膜处于修复状态,仅有少量中性粒细胞浸润,仍可见到阿米巴存在,组织内MMP-13呈弱阳性表达,平均光密度值为0.1875±0.0299,FGF2表达增强,平均光密度值为0.3735±0.0562;病程大于半年以上,角膜基质层可见大量新生血管,MMP-13及FGF2均呈微弱表达,平均光密度值分别为0.1575±0.0568、0.0363±0.0781,未见阿米巴包囊。结论阿米巴角膜炎感染早期药物治疗不理想,应及时手术清除病灶,可以有助于控制病情,利于组织修复。
Objective To observe the histopathologic changes of corneal tissues and expression of matrix metalloproteinase 13（MMP-13） and fibroblast growth factor 2（FGF2） in acanthamoeba keratitis（AK） patients.Methods Pathological changes and immunohistochemical characteristics of eight corneal tissues from patients suffering from AK were retrospectively reviewed by conventional HE staining and anti-MMP-13,anti-FGF2 immunohistochemistry method.The pathologic changes and expression of MMP-13 and FGF2 were observed.Results In patients whose course within 1 month,the corneal stoma were necrosed,and the infiltration of neutrophilic granulocyte were seen,and many cysts and trophczoites in corneal tissues were observed;Strong positive expression MMP-13（mean OD value was 0.308 5±0.078 1）and weak positive expression of FGF2（mean OD value was 0.205 7±0.038 7） were observed.In patients whose course from 1 month to 6 months,the corneal tissues were restored,and only a few neutrophilic granulocyte could be found,small amount of cysts were seen in the stroma;Strong positive expression of FGF2 （mean OD value was 0.373 5±0.056 2）and weak positive expression of MMP-13（mean OD value was 0.187 5±0.029 9）were observed.In patients whose course more than 6 months,many new vessels were seen in corneal stroma,and no cyst was found;Expression of MMP-13 （mean OD value was 0.157 5±0.056 8）and FGF2 （mean OD value was 0.036 3±0.078 1）were weak.Conclusion The early drug treatment of AK has no ideal effect,the focus of infection should be surgical removed in time,which is helpful to control the disease and restore the tissues.
出处
《眼科新进展》
CAS
北大核心
2010年第9期837-840,共4页
Recent Advances in Ophthalmology
