摘要
目的:建立能同时测定大鼠血浆中马钱子碱和士的宁浓度的HPLC分析方法。方法:大鼠血浆样品以石杉碱甲为内标,液液萃取法处理后用Kromasil C18柱分离,以乙腈-0.01 mol.L-1庚烷磺酸钠与0.02 mol.L-1磷酸二氢钾等量混合后用10%磷酸调体系pH 2.8(24∶76)作流动相,检测波长264 nm。结果:血浆中杂质不干扰样品的含量测定,马钱子碱和士的宁在50～2 000μg.L-1线性良好,平均提取回收率均大于86%,日内、日间精密度均小于8%,稳定性符合体内药物分析要求。结论:方法灵敏度高、精密度好,可应用于马钱子总生物碱中主要成分的药物动力学研究。
Objective：To establish an effective method for simultaneously determining the concentration of brucine and strychnine in rat plasma by HPLC.Method：The processed rat plasma samples were separated on a Kromasil C18 column.The mobile phase consisted of acetonitrile-0.01 mol.L-1 sodium heptane sulfonate and 0.02 mol.L-1 potassium dihydrogen phosphate,adjusted pH to 2.8 with 10% phosphonic acid（24∶ 76）.The detection wavelength was set at 264 nm.Result：Chromatograms showed endogenous peaks not interfering with the analysis.The calibration curves of brucine and strychnine were both in good linearity within the range of 50-2 000 μg.L-1.The mean extraction recoveries were more than 86% while the precisions were less than 8%.The results of stability experiment meet certain requirements for the biopharmaceutical analysis.Conclusion：The method with good sensitivity and precision is applicable to the pharmacokinetic studies of the main compounds in total alkaloids from seeds of Strychnou nux-vomica.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第11期26-29,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(30701111)
