摘要
采用单因素试验、正交试验设计对产纤维素酶的里氏木霉RutC—30菌株进行了液体摇瓶发酵条件优化实验。结果表明,在pH为4.8、每50 mL发酵液接种2.5 mL菌种时,里氏木霉RutC—30菌株产酶发酵最优培养条件是:工业纤维素30 g/L、(NH4)2SO412 g/L、Mandels营养盐浓缩液125 mL/L。在此条件下,发酵产生的纤维素酶滤纸酶活达到4.845 U.m L-1,相对于微晶纤维素碳源提高了49.6%。同时,在本实验中还发现里氏木霉RutC—30菌株的生长与产酶存在着偶联性。通过优化实验,里氏木霉RutC—30菌株达到了比较高的产纤维素酶能力,为纤维素酶进一步工业化生产奠定了一定基础。
The liquid shake-flask culture conditions of Trichoderma reesei Rut C-30 strains producing cellulase were optimized through single factor experiment and orthogonal experimental.Results show that under the precondition of pH 4.8,inoculation quantity 2.5 mL per 50 mL fermented filtrates,the optimal cultivating formula for producing enzyme was industrial cellulose 30 g/L plus（NH4）2SO4 12 g/L and Mandels nutrient solution 125 mL/L.With the formula,filter paper enzyme activity of the cellulose enzyme produced by fermenting was up to 4.845 U·mL-1 and increased by 49.6% compared to MCC.Meanwhile,it was also found that there was a coupling relationship between the growth and producing enzymes of Trichoderma reesei Rut C-30 strains.Through the optimization experiment,Trichoderma reesei Rut C-30 strains obtained higher ability of producing cellulase,which laid a foundation for cellulase＇s further industrial production.
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2010年第9期112-119,共8页
Journal of Central South University of Forestry & Technology
基金
国家"948"项目(2006-4-123)
中南林业科技大学大学生创新实验项目(2008-8D-GSX)
关键词
纤维素酶
里氏木霉Rut—30
发酵优化
滤纸酶活
正交实验设计
cellulase
Trichoderma reesei Rut C-30
optimization of fermentation
filter paper enzyme activity（FPA）
orthogonal experimental design
