摘要
目的探讨载脂蛋白Ｅ（ａｐｏＥ）基因多态性与中青年动脉粥样硬化性脑梗塞的关系。方法应用聚合酶链反应－限制性长度多态性技术（ＰＣＲ－ＲＦＬＰ），检测４３例中青年动脉粥样硬化性脑梗塞（ＡＣＩ）患者的ａｐｏＥ基因型，并与６０名同龄健康对照组比较。同时检测血脂、脂蛋白及部分载脂蛋白。结果中青年ＡＣＩ组ａｐｏＥ基因型ε３／４的频率为３０．２％，明显高于对照组（Ｐ＜０．０５）。中青年ＡＣＩ患者中ε３／３和ε３／４亚组血清Ｌｐ（ａ）均明显高于对照组（Ｐ＜０．０５），而高密度脂蛋白低于对照组（Ｐ＜０．０５）。结论ａｐｏＥ基因型ε３／４与中青年ＡＣＩ的发生相关，等位基因ε４可能是中青年ＡＣＩ的一种遗传易感因子。
Objective To study the relationship between the polymorphisms of apolipoprotein E (apoE) gene and atherosclerotic cerebral infarction (ACI) in middle aged and young adults. Methods The polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) technique were used to detect apoE genotype in 43 ACI patients in middle aged and young adults and 60 age matched controls .Meanwhile the serum levels of lipids, lipoprotein and partial apolipoprotein were determined. Results The frequency of genotype ε3/4 was 30.2% in the ACI group, which was significantly higher than that in the control group( P <0.05). The serum level of Lp(a) in the ε3/4 and ε3/3 subgroup was significantly higher than that in the control group( P <0.05),while the serum level of HDL was lower. Conclusion ApoE genotype ε3/4 may contribute to the development of ACI in middle aged and young adults and allele ε4 seemed to be a genetic susceptible factor.
出处
《临床神经病学杂志》
CAS
1999年第3期134-136,共3页
Journal of Clinical Neurology
关键词
载脂蛋白E
多态性
动脉粥样硬化性
脑梗塞
Apolipoprotein E Polymorphism Atherosclerotic cerebral infarction
