2Aleixo M J, Ferreira M L, Antunes F. Brucellosis [J]. Acta Med Port,1999,12(12):323-330.
3Office International des Epizooties. Manual of Standards for Diagnostic Tests and Vaccines. List A and B Diseases of Mammals, Birds and Bees [M]. 4th ed. Paris:Food and Agricultural Organization of the United Nations,1996.51-58.
4Richtzenhain L J, Cortez A, Heinemann M B, et al. A multiplex PCR for the detection of Brucella spp. and Leptospira spp. DNA from aborted bovine fetuses [J]. Veterinary Microbiology,2002,87(2):139-147.
5Bricher B J. PCR as a diagnostic tool for brucellosis [J].Veterinary Microbiology,2002,90:435-446.
6Andrea B, Bantle J A, Shirley M H,et al. Preliminary development of a diagnostic test for Brucella using polymerase chain reaction [J]. Journal Applied Bacteriology,1990,69:216-227.
7Edwards M J. Cattle Diseases [M]. Australia:Angus & Robertson Publisher,1984.
8Hamdy M E R, Amin A S. Detection of Brucella species in the milk of infected cattle, sheep, goats and camels by PCR [J]. The Veterinary Journal,2002,163:299-305.
9Nancy P R, Geert J, Marina V A, et al. Direct detection of Brucells spp.in raw milk by PCR and reverse hybridization with 16 S-23 S rRNA spacer probes [J]. Applied and Environmental Microbiology,1996,62(5):1683-1688.
10Diana S L, Irma O M, Ahide L, et al. Single-step PCR for detection of Brucella spp.from blood and milk of infected animals [J]. Journal of Clinical Microbiology,1995,33(12):3087-3090.