摘要
海水富营养化影响微藻生长,引起海洋初级生产力变化。以海洋小球藻(Chlorellasp.)为研究对象,研究不同磷浓度对其叶绿素荧光、细胞密度和色素含量变化的影响,以期找到海洋小球藻最适生长的磷浓度,为富营养条件下微藻生长的研究提供基础资料。结果表明,在培养温度为(22±1)℃、光照强度为4000lx、光暗比为12h∶12h条件下,不同磷浓度对海洋小球藻叶绿素荧光、细胞密度及色素含量影响显著,最大光能转化效率(Fv/Fm)、潜在活性(Fv/Fo)、实际光能转化效率(ΦPSⅡ)和电子传递速率(ETR)均呈先升后降的趋势,3.62μmol/L处理组显著低于其他各组,434.52μmol/L处理组一直处在较高水平。说明海洋小球藻的光合作用对高磷的适应范围较广,但对低磷浓度培养反应敏感。非光化学淬灭(qN)的值,3.62μmol/L处理组高于其他各组。从细胞密度和色素含量的变化可以看出,36.21μmol/L处理组最高,434.52、3.62μmol/L处理组均低于36.21μmol/L处理组。海洋小球藻生长的最适磷摩尔浓度为36.21μmol/L。
The eutrophication had significant effects on the growth of marine algae which could induce the variation of ocean primary production. In this paper, Chlorella sp. was selected as target marine algae and cultured in the medium containing different P concentration, the effects of P concentration on variation of chlorophyll fluorescence, cell density and pigment content of Chlorella sp. were investigated. The Chlorella sp..was cultured under the light intensity of 4 000 lx, light/dark cycle of 12 h/12 h and （22±1） ℃ for 7 days, Fv/Fm, Fv/Fo, ФPSⅡ and ETR of the marine algae all presented the tendency of increased first and then decreased during the cultivation. P concentrations had significant effects on the photosynthesis and growth of Chloerlla sp. （P〈0. 05）, the 4 parameters were comparatively low in group containing lower P concentration（3.62 μmol/L）, while this group obtained the highest qN value. Cell density and pigment content were the highest in group containing 36.21 μmol/L of P, multiple comparison tests showed that the optimal P contentrations for the growth of Chlorella sp. were 36.21 μmol/L.
出处
《环境污染与防治》
CAS
CSCD
北大核心
2010年第8期20-24,50,共6页
Environmental Pollution & Control
基金
国家"863计划"项目(No.2007AA09Z432)
关键词
海洋小球藻
叶绿素荧光
磷浓度
Chlorella sp.
chlorophyll fluorescence
P concentration
