摘要
采用循环伏安法探讨了细胞色素c(Cyt c)在纳米Mn3O4修饰玻碳电极表面的直接电化学行为。实验结果表明,Cyt c在纳米Mn3O4修饰玻碳电极上呈现一对峰形较好且准可逆的氧化还原峰,其式电位(E0)为56 mV,峰电流与扫描速度呈线性关系,表明电极过程是一个表面控制过程,电化学反应效率常数(ks)为4.25 s-1,固定在Mn3O4上的Cyt c能促进过氧化氢的催化还原,其实验结果可为新型传感器的研制提供依据。
The direct electrochemistry of cytochrome c（Cyt c） immobilized on Mn3O4 nanoparticle-modified glassy carbon electrode was investigated by cyclic voltammetry（CV）.The results showed that a pair of well-defined quasi-reversible redox peaks of Cyt c was obtained at Nafion/Cyt c/Mn3O4/GCE based enzyme electrode by the direct electron transfer between the protein and the electrode.The peak current was linearly dependent on the scan rate,indicating that the direct electrochemistry of Cyt c in that case was a surface-controlled process.The Cyt c immobilized on Mn3O4 can promote catalytic reduction of hydrogen peroxide.The experimental results can provide a basis for a new type of sensor.
出处
《化学试剂》
CAS
CSCD
北大核心
2010年第7期577-579,651,共4页
Chemical Reagents
基金
国家自然科学基金资助项目(20671038)
江苏省教育厅自然科学基金项目(06KJB150011)
江苏省低维材料化学重点建设实验室开放课题资助项目(JSKC09068)
