摘要
目的：研究维甲酸处理未分化外胚间充质细胞后，细胞增殖和细胞凋亡的变化，探讨细胞增殖、凋亡与维甲酸之间的关系。方法：用５μｍｏｌ／Ｌ维甲酸处理未分化外胚间充质细胞，绘制维甲酸处理前后细胞的生长曲线，并用ＴＵＮＥＬ法检测细胞凋亡数量的变化。结果：维甲酸处理未分化外胚间充质细胞后，细胞增殖受到抑制，细胞凋亡数量显著增加。结论：维甲酸可明显抑制未分化外胚间充质细胞的增殖。
objective: To study the effects of retinoic acid(RA) on the proliferation and apoptosis of undifferentiated ectomesenchymal cells derived from facial primordia of mouse fetus.Methods: Cultured undifferentiated ectomesenchymal cells were exposed to 5 μmol/L of RA, the growth and apoptosis of the cells were examined with cell counting and TUNEL. Results: The doubling time (h) of the RA treated and the control cells were 47.34 and 29.04 respectively, the TUNEL positive ratio(%) 39.18 and 4.76( P <0.01). Conclusion: RA induces apoptosis and therefore inhibits the proliferation of undifferentiated ectomesenchymal cells.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
1999年第1期47-48,共2页
Journal of Practical Stomatology
