摘要
以静态吸附与解吸为考察指标,利用五因素四水平的正交试验对AB-8型大孔树脂分离纯化枸骨叶总黄酮的工艺进行研究。结果表明,AB-8型大孔树脂对枸骨叶总黄酮有较好的纯化效果,最佳纯化工艺条件为吸附液pH4.5、吸附时间4h、解吸液体积与树脂质量之比为25:1(mL/g)、解吸液为体积分数90%的乙醇、解吸时间2.25h,在此条件下,总黄酮回收率可达90.8%,纯度为33.4%。
The application of AB-8 macroporous resin for the separation and purification of crude total flavonoids extract from Folium Ilicis Cornutae was evaluated in the present study.The static adsorption-desorption process of Folium Ilicis Cornutae total flavonoids on this resin was optimized using single factor and orthogonal array design methods.The results showed that a good separation and purification of crude total flavonoids extract from Folium Ilicis Cornutae was achieved using AB-8 macroporous resin.The optimum conditions for the separation and purification of crude total flavonoids extract from Folium Ilicis Cornutae were found to be：4 h adsorption at pH 4.5 of the sample solution,followed by 2.25 h desorption using 90% ethanol as desorption solvent added at 25-fold volume of resin weight.Under these optimum conditions,the recovery of Folium Ilicis Cornutae total flavonoids reached up to 90.8% and the purity of the final product obtained was 33.4%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第12期76-79,共4页
Food Science
基金
湖南省教育厅科学研究项目(08C920)
中南林业科技大学林木遗传育种学科资助项目(0014)
关键词
枸骨叶
总黄酮
AB-8大孔树脂
分离纯化
Folium Ilicis Cornutae
total flavonoids
AB-8 macroporous resin
separation and purification
