摘要
应用高效液相色谱-电喷雾离子阱质谱法鉴定了复方酸枣仁汤中的化学成分。实验采用Zorbax-C18色谱柱,二元线性梯度洗脱进行色谱分离,并与电喷雾离子阱质谱联用,根据负离子模式下的分子量与裂解规律,通过与文献及部分对照品的比对,推测化合物可能的结构。首次建立了高效液相色谱-电喷雾离子阱质谱法测定酸枣仁汤中化学成分及其在大鼠血浆中的吸收代谢成分,对进一步研究复方酸枣仁汤有较重要的意义。
Chemical components of Fufang Suan-Zao-Ren-Tang decoction and their metabolites in rats were identified by HPLC-ESI-MS.Samples were separated by a Zorbax C18 column using a binary gradient elution,and analyzed by HPLC-ESI-MS.The ions of compounds in negative modes were observed for molecule mass information,and the potential structures were identified by attentive studying on the mass spectra of compounds and comparing with reference data and some of standards.It is the first time to establish an analysis method using HPLC-MC to identify the chemical and metalobic components of Fufang Suan-Zao-Ren-Tang decoction.Further research about Suan-Zao-Ren-Tang decoction can base on this analysis method.
出处
《药学与临床研究》
2010年第3期250-252,共3页
Pharmaceutical and Clinical Research
基金
"重大新药创制"科技重大专项(2009ZX09502-011)
关键词
高效液相色谱-电喷雾离子阱质谱联用技术
复方酸枣仁汤
吸收代谢成分
High performance liquid chromatography-electro spray ion trap-mass spectrometry
Suan-Zao-Ren-Tang decoction
Chemical components
Metabolites
