摘要
目的 :应用环磷酰胺诱导小鼠对心肌肌球蛋白免疫耐受 ,探索免疫耐受在心肌炎和扩张型心肌病治疗中的作用。方法 :实验组 ( n =2 0 )于实验第 1,14和 2 8天 3次以猪心肌球蛋白免疫 ,于第1次免疫 2 4h后腹腔注射环磷酰胺 2 2 0 mg/ kg;对照组 ( n =2 0 )按同样方法免疫 ,第 1次免疫 2 4h后以等体积生理盐水代替环磷酰胺腹腔注射。第 35天采集小鼠血清和取心脏标本 ,EL ISA法检测小鼠血清中抗肌球蛋白抗体 ,显微镜下观察心肌病变。结果 :实验组未检测出抗肌球蛋白抗体 ,对照组17例抗体阳性 ,阳性率 85 .5 %。病理结果显示对照组小鼠可见明显炎症病灶 :血管周围炎及心肌间质淋巴细胞、单核细胞浸润。实验组小鼠未见以上病变。结论 :肌球蛋白可以引起小鼠免疫反应及心脏炎性改变 ;环磷酰胺可诱导小鼠对肌球蛋白产生免疫耐受 ,防止心肌损伤。
Objective:The immune tolerance to cardiac myosin was induced by cyclophosphamide in mouse,in order to research the action of immune tolerance in therapy of myocarditis and dilated cardiomyopathy.Method:The mice of test group (n=20) were immunized with cardiac myosin at 1 d,14 d and 28 d respectively,and cyclophosphamide was injected intraperitoneally at 24 hours after the first immunization.The mice of control group (n=20) were immunized as same as the test group,but cyclophosphamide wase replaced by physiological saline with the same volume.The serum and heart sample of the mice were collected at 35 d.The antibodies against cardiac myosin were examined with ELISA and pathological myocardium were observed in microscope.Result:The antibodies against cardiac myosin were negative in the test group,and positive in 17 samples of the control group,positive rate was 85.5 %.The inflammatory focus including inflammation around vessels,infiltration of mononuclear and lymphocyte in myocardial mesenchyma was observed in the control group,and the above pathologic changes was not seen in the test group.Conclusion:The cardiac myosin injection might result in the immune response and cardiac inflammatory reaction in mouse,the immune tolerance to cardiac myosin might be induced by cyclolphosphamide and myocardial injury might be prevented in mouse.
出处
《临床心血管病杂志》
CAS
CSCD
北大核心
1999年第1期24-26,共3页
Journal of Clinical Cardiology
关键词
环磷酰胺
肌球蛋白
免疫耐受
心肌病
Cyclophosphamide Myosin Immune tolerance Myocarditis Dilated cardiomyopathy
