摘要
研究建立气相色谱法测定茶叶中氟虫腈残留量的方法。样品用乙腈提取,弗罗里硅土(含活性炭)柱层析净化,配有μECD检测器的气相色谱仪测定。氟虫腈的最小检测限量为2×10-12g,最低检测限为2μg/kg。茶叶中氟虫腈的添加回收率为74.0%～92.0%,变异系数为3.26%～10.55%。方法的准确性、灵敏度均达到农残分析的要求。
An analytical method based on gas chromatography （GC） was proposed for the determination of fipronil residue in tea. Samples were extracted with acetontrile and cleaned up on a Florisil （containing graphitized carbon black） column prior to HPLC analysis with μECD detection. The minimum detectable amount of fipronil was 2 × 10-12 g and the minimum detectable concentration of fipronil in tea was 2μg/kg. Average recoveries of 3 replicates for fipronil in a real sample spiked at 5 levels were within the range of 74.0%-92.0%, with a RSD ranging from 3.26% to 10.55%. The accuracy and sensitivity of the developed method both meet the requirements of pesticide residue analysis.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第10期279-282,共4页
Food Science
关键词
氟虫腈
气相色谱法
茶叶
固相萃取
fipronil
gas chromatography
tea
solid-phase extraction
