摘要
为研究广东省诺如病毒胃肠炎暴发疫情的分子流行病学特点,我们采集了2005～2008年期间24起急性暴发性胃肠炎患者的粪便和肛拭子标本,使用诺如病毒特异性引物,通过逆转录-聚合酶链反应(RT-PCR)技术进行检测,再经核苷酸序列测定以分析诺如病毒的基因型,同时收集急性暴发性胃肠炎患者的相关流行病学资料。结果显示:24起急性暴发性胃肠炎中19起由诺如病毒引起,时间主要集中在每年的10月至次年2月,2005年病毒胃肠炎暴发疫情是由GⅡ-3基因型病毒引起,主要为幼儿园和小学,发生地主要在内陆山区,2006年秋季疫情以后则均为GⅡ-4型的变异株2006b引起,主要为大学和社区,2007年疫情数比其他年份高1倍,发生地遍及广东全省。广东省诺如病毒变异株2006b在个别特殊的基因位点呈现出高度的地域一致性。随着诺如病毒流行株的基因型由GⅡ-3变为GⅡ-4型,广东省诺如病毒流行的强度大大增加,新出现诺如病毒变异株2006b引起的暴发波及地方多,涉及人群从低幼儿童为主扩展到全年龄组,表明GⅡ-4新变异株比其它毒株具有更高的侵袭力。
To study the molecular epidemiological characteristics of norovirus gastroenteritis outbreaks in Guangdong,we collected fecal and anal swabs specimens from 24 outbreaks of acute gastroenteritis from 2005 to 2008 to detect norovirus. Specimens were detected by RT-PCR and then sequenced. The descriptive data were also collected. According to our research,19 of 24 outbreaks of gastroenteritis were positive for norovirus. The occurrence time was from October to next February mainly. The strains in 2005 belonged to GⅡ-3 genotype and all outbreaks occurred in kindergarten and school. But from autumn of 2006,the outbreaks were all caused by GⅡ-4/2006b variant and occurred in universities and community. The number of outbreaks in 2007 increased greatly and covered all over province. The nucleotide sequences of Guangdong strains in some sites showed high regional identity. Our results showed that with the shift of genotype from GⅡ-3 to GⅡ-4,occurrence of norovirus outbreaks increased greatly. The outbreaks of norovirus caused by GⅡ-4/2006b variant spreaded widely and the involved population covered children and adult,indicating the strong invasiveness of this variant.
出处
《病毒学报》
CAS
CSCD
北大核心
2010年第3期202-207,共6页
Chinese Journal of Virology
基金
广东省医学科学基金项目"广东省诺如病毒分子流行病学研究"(A2007065)
关键词
诺如病毒
流行病学
暴发
分型
norovirus
epidemiology
outbreak
genotyping
