摘要
采用微卫星标记进行缢蛏(Sinonovacula constricta)群体遗传研究时,群体的样本量多少对遗传多样性指标准确性有影响。实验设置了11个样本量梯度,并对观测等位基因数(Na),有效等位基因数(Ne),表观杂合度(Ho),期望杂合度(He)和Nei’s遗传多样性指数(H)的变化趋势进行了分析。结果表明,样本量与观测等位基因数和有效等位基因数具有高度的正相关性,与杂合度和内氏遗传多样性指数呈中度相关。当样本量小于20时,遗传多样性指标均呈现迅速增加的趋势;当样本量大于30,杂合度和内氏遗传多样性指数趋于稳定。建议在利用微卫星进行群体遗传结构评估时,最适参数为期望杂合度和内氏遗传多样性指数,最小样本量为30。
When the microsatellite marker is used to study the population genetics of Sinonovacula constric- ta the population sample size may effect on the accuracy of the genetic diversity indices. Totally 11 grades of sample size are designed in the experiment to analyze the variabilities caused by the sample size grades and given to the observed number of alleles per locus （Na）, effective number of alleles per locus （Ne）, observed Heterozygosity （Ho）, Expected Heterozygosity （He） and Nei＇s genetic diversity index （H）. The results show that sample size is highly related to Na and Ne, and mediately to Ho, He, and H. When the samples are less than 20 individuals, all the genetic diversity indices tend to be increased rapidly. When the sample size exceeds 30, He and H tend to be stable. It is suggested that the minimum sample size is 30 and the most suitable indices are He and H in using microsatellite markers to estimate the population genetic diversity of Sinonovacula constricta.
出处
《海洋科学进展》
CAS
CSCD
北大核心
2010年第2期203-208,共6页
Advances in Marine Science
基金
国家高技术研究发展计划项目--缢蛏高产
抗逆品种培育(2006AA10A410)
福建省海洋与渔业局重点项目--缢蛏优良品种选育与高产养殖技术研究(闽海渔2007015)
关键词
缢蛏
微卫星标记
样本量
遗传多样性指标
Sinonovacula constricta
microsatellite marker
sample size
genetic diversity index
