摘要
以坪山柚(Citrus grandisOsbeck‘Pingshanyou’)无菌苗的上胚轴为外植体,研究了其离体培养和植株再生技术。结果表明:坪山柚上胚轴的形态学上部出芽能力最强,诱导不定芽的最佳培养基为:MS+6-BA 1.0 mg L-1+TDZ 0.05 mg L-1+NAA0.2 mg L-1+GA31.0 mg L-1+蔗糖40 g L-1,培养4周后不定芽诱导率为100%,平均不定芽数为8.35;最适生根培养基为1/2MS+NAA1.5 mg L-1,生根率为88.7%,平均生根6.4条;生根试管苗移栽30 d后成活率达90%。
The plant regeneration in vitro was studied using epicotyl segments of Citrus grandis Osbeck ＇Pingshanyou＇ as explants.The results showed that the optimum explants were the upper segments of epicotyl,the optimum medium for adventitious shoots induction was MS＋6-BA 1.0 mg L-1＋TDZ 0.05 mg L-1＋NAA 0.2 mg L-1＋GA3 1.0 mg L-1＋ sucrose 40 g L-1,the induction rate was 100% and the average adventitious shoots reached 8.35 after cultured for 4 weeks.The optimum medium for rooting was 1/2MS＋NAA 1.5 mg L-1,the rooting and the average roots were 88.7% and 6.4,respectively.The plantlet survival was 90% after transplanted for 30 days.
出处
《热带亚热带植物学报》
CAS
CSCD
北大核心
2010年第3期298-303,共6页
Journal of Tropical and Subtropical Botany
基金
四川省教育厅项目(2003A175)
四川省绵阳市科技攻关项目(2005BN001-1)资助
关键词
坪山柚
离体培养
植株再生
Citrus grandis Osbeck ＇Pingshanyou＇
Culture in vitro
Plant regeneration
