摘要
目的研究表没食子儿茶素没食子酸酯(EGCG)对急性酒精中毒小鼠神经行为学变化及血醇浓度的影响。方法昆明种小鼠随机分为6组,正常组、模型组、EGCG25、75、225mg/kg三个剂量组、阳性药组(葛根胶囊180mg/kg)。给药组灌胃给予相应药物,模型组给予等量生理盐水,0.5h后,按5g/kg灌胃市售56%(v/v)二锅头酒,在0.25、0.5、0.75、1、2h时分别检测各组小鼠神经行为学指标(自主活动、Morris水迷宫、转棒实验)及血醇浓度。结果与模型组比,EGCG75、225mg/kg剂量组在0.25～1h自主活动次数降低,在0.75～2h乙醇小鼠的平衡失调有显著缓解,在0.5～1h血醇浓度降低,EGCG225mg/kg剂量组显著改善乙醇小鼠的空间辨别能力。结论 EGCG可降低血醇浓度,抑制乙醇小鼠中枢神经系统的兴奋性,改善乙醇所致的小鼠空间辨别障碍及共济失调现象。
Objective To investigate the effects of epigallocatechin-3-gallate（EGCG） on the neurobehavioral appearance and blood alcohol concentration of acute alcoholism in mice.Methods Mice were randomly divided into 6 groups：normal group,model group,EGCG（25,75,225 mg/kg） treatment group and Ge Gen capsule（180 mg/kg） treatment group.Mice in the model group were treated with the same saline and mice in the treatment groups were administered with the related drug.After 0.5 h,all groups were infused 56%（v/v）＂Er Guo Tou＂with 5 g/kg.Then locomotors activity,Morris water maze,balance beam test and blood alcohol concentration were detected at 0.25,0.5,0.75,1 and 2 h after infused alcohol.Results Compared with model group,EGCG（75,225 mg/kg） had significantly decreased the locomotors activities from 0.25 to 1 h,moderated ataxia from 0.75 to 2 h and decreased blood alcohol concentration from 0.5 to 1 h.Especially,EGCG（225 mg/kg）had significantly improved spatial discrimination disorders.Conclusion EGCG can decrease blood alcohol concentration,suppress the irritability of central nerve system and improve spatial discrimination disorders and ataxia.
出处
《安徽医科大学学报》
CAS
北大核心
2010年第3期342-345,共4页
Acta Universitatis Medicinalis Anhui
基金
安徽省研究实验基地优秀中青年科研带头人基金(编号:200622805)
关键词
酒精中毒
急性/药物疗法
神经行为学表现
alcoholic intoxication
acute/drug therapy
neurobehavioral manifestations
