摘要
目的测定子宫肌瘤和卵巢癌患者血清自体荧光光谱,探讨子宫肌瘤和卵巢癌的特征自体荧光光谱,为子宫肌瘤和卵巢癌的光谱学诊断提供理论依据。方法应用荧光分光光度计,在301nm的激发光激发下,对23例子宫肌瘤患者、17例卵巢癌患者和40例正常人的血清进行了测定,对其荧光光谱的峰位置、峰面积、峰高及主峰间的比值进行了分析。结果子宫肌瘤组在335nm附近发生了紫移(P<0.05),卵巢癌组在540nm附近明显红移(P<0.05)。子宫肌瘤组及卵巢癌组在302nm附近的峰高明显升高(P<0.05)。子宫肌瘤和卵巢癌患者的血清自体荧光光谱与正常人有显著的差异(P<0.05),子宫肌瘤和卵巢癌患者有其特征的荧光光谱。结论应用血清自体荧光光谱对子宫肌瘤和卵巢癌患者进行诊断具有重要的参考价值。
Objective Measure auto - fluorescent spectrum of serum for Hysteromyoma or oophoroma diseases, explore the characteristic spectrum of different Hysteromyoma or oophoroma diseases, in order to supply theoretical proof for spectroscopic diagno- sis for Hysteremyoma or oophoroma diseases. Methods Measure auto - fluorescent spectrum of serum for 23 cases of Hysteromyoma, ,17 cases of oophoroma and 40 normal people with wavelength 301nm by fluorospectrophotometer, then analyze fluorescent spectrum of the peak positions, areas, heights and ratio of main peak. Results Hysteromyoma group move violet shift with wavelength 335 nm. （ P 〈 0.05 ）, oophorema r group move red shift （ P 〈 0.05 ） with wavelength 540 nm obviously, the peak heights of Hysteromyoma or oophoroma go up a lot with wavelength 302nm（ P 〈 0.05） obviously. So there are obvious significant difference between auto - fluorescent spectrum of serum for gynecologic carcinoma and normal people, therefore, Hysteromyoma or oophoroma group has characteristic fluorescent spectrum. Conclusion It has important value that making Hysteromyoma or oophoroma people＇s diagnosis through measureing auto - fluorescent spectrum of serum for Hysteromyoma or oophoroma diseases .
出处
《河南预防医学杂志》
2010年第3期174-176,共3页
Henan Journal of Preventive Medicine
关键词
子宫肌瘤和卵巢癌
血清
荧光光谱
光谱分析
Hysteromyoma or oophoroma
Serum
Fluorescent spectrum
Spectrum analysis
