摘要
以虫草素含量为指标,采用正交实验优化了适合拟黑虫草(Cordyceps nigrella)菌株cnig-56固体发酵的培养基,并探讨了该菌株固体发酵的最佳条件。结果表明,菌龄为3d的拟黑虫草菌种按10%接种量接入优化的固体培养基上(配方为大米粉9.9%,青稞粉6.6%,玉米粉9.9%,蚕蛹粉3.3%,粗麸皮70.15%,KH2PO40.05%,MgSO40.1%)于24℃发酵培养30d,其产物的虫草素含量最高为101μg/g。
Orthogonal testing was used to optimize selected parameters affecting cordycepin production by Cordyceps nigrella （strain cnig-56） grown under solid-state conditions. Highest cordycepin levels （101 μg/g） were recorded after 30 d cultivation at 24 ℃ on a medium consisting of 9.9% rice powder,6.6% highland barley powder,9.9% corn powder,3.3% dried silkworm chrysalis powder,70.15% unrefined wheat bran,0.05% KH2PO4,0.1% MgSO4·7H2O,a solid：water ratio of 1：1.2,and using 3-day-old mycelium （10% w/w） as inoculum.
出处
《食用菌学报》
北大核心
2010年第1期80-82,共3页
Acta Edulis Fungi
基金
云南省自然科学基金重点项目(编号:2008CC019)的部分研究内容
关键词
拟黑虫草
固体培养
正交实验
虫草素
Cordyceps nigrella
solid fermentation
orthogonal test
cordycepin
