摘要
从以C15—C36直链烷烃为惟一碳源生长的解烃菌——地芽孢杆菌MD-2细胞中获得了1个新的烃降解基因——烷烃单加氧酶基因sladA。将基因sladA克隆到质粒pSTE33上,构建了重组质粒pSTalk。通过电转化将pSTalk转化入嗜热脱氮土壤芽孢杆菌ZJ-3内,构建了基因工程菌SL-21。SL-21兼具嗜热和解烃的功能,在70℃条件下,14d后对原油的降解率达75.08%。研究结果表明,可以通过体外重组的方式向嗜热菌中引入烃降解基因,从而构建嗜热解烃基因工程菌。
A new alkane oxygenase,slad A,was obtained in a hydrocarbon degrading strain,Geobacillus sp.MD-2,which could grow on n-alkanes (from C15to C36) as the sole carbon source.The gene slad A was inserted into plasmid pSTE33 as recombined plas-mid pSTalk.The plasmid pSTalk was transformed into thermophilic Geobacillus thermodenitrificans ZJ-3,and a genetically en-gineered bacterium SL-21 was constructed.SL-21 can degrade 75.08% of the hydrocardon after 14d at 70 C,and it has the both functions of thermophilic living and hydrocarbon degrading.The results indicate that the thermophilic genetically engi-neered bacterium can be constructed by importing hydrocarbon-degrading gene into the thermophilic bacterium using DNA re-combmant in vitro.
出处
《油气地质与采收率》
CAS
CSCD
北大核心
2010年第1期80-82,共3页
Petroleum Geology and Recovery Efficiency
基金
中国石化科技攻关项目"高含水油藏微生物驱油技术研究"(P05076)
中国石化股份胜利油田分公司科技攻关项目"石油微生物基因工程菌研究"(YKS0604)
关键词
微生物采油
烃类降解菌
嗜热解烃基因
质粒
基因工程菌
MEOR
hydrocarbon degrading strain
thermophilic gene for hydrocarbon degrading
plasmid engineered bacterium
