摘要
目的观察先天性心脏病患者体外循环围手术期血管性假血友病因子（ｖｏｎＷｉｌｅｂｒａｎｄｆａｃｔｏｒ，ｖＷＦ）及血循环内皮细胞（ｃｉｒｃｕｌａｔｉｎｇｅｎｄｏｔｈｅｌｉａｌｃｅｌｓ，ＣＥＣ）数的动态变化过程，探讨低温体外循环对血管内皮细胞的影响。方法于肝素化后体外循环前、转流３０分钟、开放主动脉５分钟、停机、停机后４小时和术后第１天晨用酶联免疫法测定２０例先天性心脏病患者血浆中ｖＷＦ浓度，同时用Ｐｅｒｃｏｌ密度梯度离心法对患者血中ＣＥＣ进行计数和形态学观察。结果转流３０分钟、开放主动脉５分钟、停机、停体外循环后４小时、术后第１天晨ｖＷＦ水平和ＣＥＣ数较体外循环前显著升高（Ｐ＜０．０５）。
Objective To observe the effect of hypothermic extracorporeal circulation(ECC) on von Willebrand factor (vWF) and circulating endothelial cells(CEC) in pediatric cardiac operations. Methods Twenty children scheduled for cardiac operation with hypothermic ECC were included in the study.vWF concentrations and CEC were measured before onset of ECC, 30 minutes after onset of ECC, 5 minutes after release of the aortic crossclamp, at the end of ECC, 4 hours after the end of ECC and on the morning of first postoperative day. Results The level of vWF and CEC increased during ECC and was remained higher than the basic values in the morning of first postoperative day. Conclusion The results indicate endothelial cells are injured during pediatric cardiac operations with hypothermic ECC.
出处
《中国胸心血管外科临床杂志》
CAS
1999年第1期10-11,共2页
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
关键词
体外循环
循环内皮细胞
假血友病因子
Extracorporeal circulation
Circulating endothelial cells
von Willebrand factor
Endothelial cell
