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全反式维甲酸诱导小鼠间充质干细胞C3H10T1/2表达碱性磷酸酶 被引量：2

All Trans Retinoic Acid Upregulates Alkaline Phosphatase in Mouse Mesenchymal Stem Cell C3H10T1/2
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摘要 目的分析全反式维甲酸对小鼠间充质干细胞C3H10T1/2表达碱性磷酸酶的影响。方法全反式维甲酸(10-6M)、维甲酸核受体RAR的激动剂Ch55与MAPK信号通路的阻滞剂处理C3H10T1/2细胞,钙钴染色检测碱性磷酸酶活性,定量RT-PCR方法检测量效关系以及时相分布;采用萤光素酶报告基因检测碱性磷酸酶的启动子是否接受维甲酸的调控。结果维甲酸处理C3H10T1/2细胞24h即能够明显上调ALP的活性,具有明显的剂量依赖特性,与RAR有关,无MAPK信号通路的参与,在其转录起始位点上游-512bp的范围内检测到核受体RAR的调控位点。结论维甲酸能够通过核受体RAR直接上调碱性磷酸酶的转录水平。 Objective To investigate the effects of all trans retinoic acid （ATRA） on the transcription level of mouse embryonic mesenchymal stem cell C3H10T1/2.Methods C3H10T1/2 were treated with all trans retinoic acid（10-6M）,retinoic acid receptor （ RAR） agonist Ch55 and MAPK signal pathway inhibitor,alkaline phosphatase（ALP）activity were monitored by ALP staining,ALP transcription level were analyzed by quantitative RT-PCR;Systemic promoter analysis based on Luciferase reporter assay was undertaken to search for ALP promoter region to which RAR binds.Results ATRA stimulates ALP activity significantly in 24 h,which is dosage dependent and relevant to RAR,and the potential RAR binding site exists in the-512bp region of ALP promoter.Conclusion ATRA upregulates ALP expression in C3H10T1/2 cells mediated by RAR.
作者 胡伶平 孙奋勇 董群伟
机构地区 广东药学院附属第一医院骨科 暨南大学生物工程研究所
出处 《中国实用医药》 2010年第13期4-6,共3页 China Practical Medicine
关键词 全反式维甲酸 间充质干细胞 碱性磷酸酶 启动子 All trans retinoic acid Mesenchymal stem cell Alkaline phosphatase Promoter
分类号 R96 [医药卫生—药理学]
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参考文献8

  • 1Weston AD,Rosen RAS,Underhill TM.Regulation of skeletal progenitor differentiation by the BMP and retinoid signaling pathways.J Cell Biol,2000,148:679-690.
  • 2Hoshi K,Ejiri S,Ozawa H.Localization alteration of calcium,phosphorus,and calcification-related organics such as proteoglycans and alkaline phosphatase during bone calcification.J Bone Miner Res,2001,16:289-298.
  • 3Kim YJ,Lee MH,John M,Cho JY,Ryoo HM.Bone Morphogenetic Protein-2-induced Alkaline Phosphatase Expression Is Stimulated by Dlx5 and Repressed by Msx2.J Biol Chem,2004,279(49):50773-50780.
  • 4Bubnoff A and Cho KWY.Intracellular BMP Signaling Regulation in Vertebrates:Pathway or Network? Developmental Biology,2001,239:1-14.
  • 5Derynck R,Zhang YE.Smad-dependent and Smad-independent pathways in TGF-b family signaling.Nature,2003,425:577-584.
  • 6Yen A,Roberson MS & Varvayanis S.Retinoic acid selectively activates the ERK2 but not JNK/SAPK or p38 MAP kinases when inducing myeloid differentiation.In Vitro Cellular and Developmental Biology Animal,1999,35:527-532.
  • 7Yen A & Varvayanis S.Retinoic acid increases amount of phosphorylated RAF ectopic expression of cFMS reveals that retinoic acid-induced differentiation is more strongly dependent on ERK2 signaling than induced GO arrest is.In Vitro Cellular and Developmental Biology Animal,2000,36:249-255.
  • 8Bost F,Caron L,Marchetti I,Dani C,Le Marchand-Brustel Y & Binetruy B.Retinoic acid activation of the ERK pathway is required for embryonic stem cell commitment into the adipocyte lineage.Biochemical Journal,2002,361:621-627.

同被引文献19

  • 1郭俊明,何小洪,罗超权.体外诱导大肠癌细胞分化及其碱性磷酸酶活性的变化[J].中国胃肠外科杂志,1999,2(1):53-55. 被引量：3
  • 2Arthur A,Zannettino A,Gronthos S. The therapeutic applications of multipotential mesenchymal/stromal stem cells in skeletal tissue repair[J].Journal of Cellular Physiology,2009,(02):237-245.doi:10.1002/jcp.21592.
  • 3Kang Q,Sun M H,Cheng H. Characterization of the distinct orthotopic bone-forming activity of 14 BMPs using recombinant adenovirus-mediated gene delivery[J].Gene Thor,2004,(17):1312-1320.
  • 4Tang N,Song W X,Luo J. BMP-9-induced osteogenic differentiation of mesenchymal progenitors requires functional canonical Wnt/beta-catenin signalling[J].Journal of Cellular and Molecular Medicine,2009,(8B):2448-2464.
  • 5Chen L,Jiang W,Huang J. Insulin-like growth factor 2 (IGF-2)potentiates BMP-9-induced osteogenic differentiation and bone formation[J].Journal of Bone and Mineral Research,2010,(11):2447-2459.
  • 6Kambhampati S,Verma A,Li Y. Signalling pathways activated by all-trans-retinoic acid in acute promyelocytic leukemia cells[J].Leukemia and Lymphoma,2004,(11):2175-2185.
  • 7Wang A,Ding X,Sheng S. Retinoic acid inhibits osteogenic differentiation of rat bone marrow stromal cells[J].Biochemical and Biophysical Research Communications,2008,(03):435-439.
  • 8Oki Y,Watanabe S,Erdo T. Mature adipocyte-derived dedifferentiated fat cells can trans-differentiate into osteoblasts in vitro and in vivo only by all-trans retinoic acid[J].Cell Structure and Function,2008,(02):211-222.
  • 9He B C,Chen L,Zuo G W. Synergistic antitumor effect of the activated PPARgamma and retinoid receptors on human osteosarcoma[J].Clinical Cancer Research,2010,(08):2235-2245.
  • 10Luo J,Deng Z L,Luo X. A protocol for rapid generation of recombinant adenoviruses using the AdEasy system[J].Nature Protocols,2007,(05):1236-1247.

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中国实用医药
2010年 第13期

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