摘要
以螺旋木霉(Trichoderma SpiraleXX)、小克银汉霉(Cunninghamella phaeospora MK)和卵形孢球托霉(Gongronella butleri XT)3种丝状真菌为材料,采用改进的CTAB法提取基因组DNA.方法改进后无需液氮、聚乙烯砒咯烷酮(PVP)和NaAc等试剂,过程简洁,且所需菌体量少,提取的DNA纯度较好,适用于一次微量提取多个样品的基因组DNA.此方法得到的基因组DNA可用于PCR扩增.
Genomic DNA of the three filamentous fungi Trichoderma Spirale XX, Cunninghamella phaeospora MK and Gongronella butleri XT were extracted with a modified Cetyltrimethyl Ammonium Bromide （CTAB） method. The improued method does not require liquid nitrogen, PVP and NaAc etc. The process was simple and efficient. The result indicated that higher purity of genomic DNA was obtained with less amount of mycelium by the method which was suitable to treat many samples at one time, and genomic DNA for basic molecular experiments such as PCR.
出处
《生命科学研究》
CAS
CSCD
2010年第2期122-124,149,共4页
Life Science Research
基金
湖南省教育厅基金资助项目(B30939)
