摘要
目的建立一种新型、可靠的脊髓压迫动物模型,为探索脊髓受压后的分子机制及临床治疗的研究奠定基础。方法根据兔椎骨及脊柱的形态特点,设计一种新型的兔脊髓压迫器,并用以制作兔脊髓急性压迫模型。运用改良Tarlov法、TTC、HE及Nissl染色验证该模型的可靠性。结果脊髓受压后,兔后肢的肌力减退、行动迟缓;TTC结果显示压迫段及其相邻节段均有不同程度的缺血,压迫后段还存在不同程度的出血及组织坏死;脊髓受压后出现组织水肿,神经元肿胀、核固缩,尼氏体减少甚至消失等病理改变。结论①用自行设计的压迫器制作兔脊髓压迫模型具有方法简便、科学、重复性强等特点;②本实验制作的兔脊髓压迫模型是用于研究脊髓压迫性损伤病理生理机制及临床治疗的理想模型。
Objective To establish a new andreliable rabbit model of compression spinal cord injury,so as to provide a basis for further studies on molecular mechanism and clinical therapy of this kind of disease. Methods A self-made device for establishing acute compressed spinal cord injury was designed according to the characteristics of rabbit spinal column and vertebrae,which included four parts： screw,plastic cannula,fixed steel board and compressed steel board on spinal cord. Postoperative behavioral and histological changes were observed. TTC,HE,Nissl staining methods were used to evaluate the effects of this model. Results The modified Tarlov behavior testing showed that,all rabbits in model group presented with weakness of muscle power in their hind legs and motor abnormalities. TTC staining showed the ischemia of diversity degrees at different segments of spinal cord,the compressed segment,the anterior segment adjacent to the compressed segment and the posterior segment adjacent to the compressed segment. In addition,the hemorrhage and tissue necrosis occurred in the posterior segment adjacent to the compressed segment; HE and Nissl staining showed the edema of neuronal tissue,karyopyknosis in some neurons and the decrease or even the disappearance of Nissl body. Conclusions The acute compressed spinal cord injury model established by our self-made device accurately replicates the characteristics of acute compressed spinal cord injury ,which is simple,replicable and adjustable.
出处
《中国临床解剖学杂志》
CSCD
北大核心
2010年第2期208-213,共6页
Chinese Journal of Clinical Anatomy
基金
国家自然科学基金资助项目(30270437)
教育部春晖计划资助(2004年)
重庆市重点教改课题资助(09-2-061)
关键词
脊髓
损伤
急性压迫模型
兔
Spinalcord
Injury
Acutecompressedmodel
Rabbit
