摘要
建立了一种新的测定Ｆｅｎｔｏｎ反应产生羟自由基·ＯＨ的方法。Ｃｅ３＋在稀硫酸中能产生特征荧光，其最大激发波长和发射波长分别为 ２８０ ｎｍ和３６０ ｎｍ。 Ｆｅｎｔｏｎ反应产生的·ＯＨ能将 Ｃｅ３＋氧化成 Ｃｅ４＋，用荧光法测定 Ｃｅ３＋的荧光强度变化即可间接测定羟自由基的产生量。通过对测定条件的研究，得到最佳实验条件。结果表明，该方法稳定性好、操作简便。测定快速，可作为一种简便的筛选抗氧化剂的方法。
A new method for detecting the hydroxyl radical produced by Fenton reaction is developed.' Ce3+ can produce its characteristic fluorescence in diluted sulfuric acid with is excitation and emission wavelength at 280 nm and 360 nm, respectively. The fluorescence is qUenched when Ce3+ is oxidized by hydroxyl radical The optimal experimental program is obtained by studying the determination conditions. The results show that this method is stable, simple and rapid. This system can be used as a convenient method for. the selection of antioxidants.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1998年第12期1460-1463,共4页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金资助课题!(2963700)
