摘要
目的分析BALB/c小鼠肝、脑组织差异基因表达谱。方法TRIZOL法提取总RNA,反转录cDNA,采用36K小鼠全基因组寡核苷酸芯片,运用荧光双交换方法分析BALB/c小鼠肝脑组织中mRNA转录本含量。经芯片扫描、图像采集后,运用MAS2.0软件对差异基因进行基因功能分析(GO)和KEGG通路分类。结果基因芯片结果表明与脑组织相比,在肝组织中共发现差异表达基因6 132个,其中包括上调基因2 974个,下调基因3 158个,GO分析上述差异表达基因主要涉及代谢、细胞信号转导、DNA结合与转录、细胞周期、细胞骨架、细胞粘附和发育等。KEGG信号通路分析显示:上调差异基因中71个通路的改变差异具有统计学意义(P<0.05),下调差异基因中有80个通路的改变差异具有统计学意义(P<0.05)。结论小鼠肝与脑组织基因表达谱的差异涉及多个基因表达调控途径,研究这些基因分子功能有助于深入了解各组织独特的功能表达系统。
Objective To identify genes differentially expressed in the liver and brain of BALB/c mice.Methods The total RNA of liver and brain tissue in BALB/c mice was extracted with TRIZOL and reverse transcripted to cDNA.mRNA transcripts from the liver and brain of BALB/c mice were analyzed by fluorescence exchange with a 36K whole mouse genome oligonucleotide microarray.After data were analyzed,genes with a threshold of a 2-fold change were selected and Gene Ontology and KEGG（Kyoto Encyclopaedia of Genes and Genomes） pathway databases were used to identify functionally related GO terms and gene pathways.Results The microarray showed that the expression levels of 6 132 genes were markedly altered,including 2 974 up-regulated genes and 3 158 down-regulated genes.GO analysis indicated that these altered genes were associated with metabolism,signal transduction,DNA binding and transcription,the cell cycle,the cytoskeleton,cell adhesion,development,and other processes.KEGG analysis showed that 71 signal pathways associated with up-regulated genes and 80 signal pathways associated with down-regulated genes had changed significantly（P0.05）.Conclusion Many genes with different functions were differentially expressed in the liver and the brain.Discovering such candidate genes may help to further study the system of tissue-specific functional expression.
出处
《中国病原生物学杂志》
CSCD
2010年第3期180-183,F0003,共5页
Journal of Pathogen Biology
基金
新疆维吾尔自治区科技支疆项目(No.200891126)
新疆包虫病基础医学重点实验室开放课题(No.XJDX0202-2008-03)
