摘要
目的：建立非抑制离子色谱法测定染发剂中铵和乙醇胺含量的方法。方法：染发剂通过用乙腈超声、静置、离心后,取上清液3 ml过石墨碳黑SPE柱加C18小柱,过0.22μm尼龙滤膜后,取滤液进样分析。染发剂在Ion Pac SCS 1分离柱（250 mm×4 mm i.d.）上,利用2 mmol/L的甲基磺酸-10%乙腈淋洗液。结果：铵和乙醇胺在0～200μg/ml范围均具有良好的线性,相关系数均大于0.999,检出限分别为1.9和2.4μg/L,回收率分别为97.8%～101.6%和95.6%～99.0%,RSD均小于5.0%。结论：建立的方法可用于染发剂中铵和乙醇胺含量的测定,结果满意。
Objective：To establish a sensitive method for the determination of ammonium and ethanolamine in hair dye by ion chromatography with non-suppressed conductivity detection.Methods：Dye sample was ultrasounically extracted with acetonitrile.After centrifugation,the supernatant was cleaned up with SPE column and C18 column and filtrated with,0.22 μm nylon membrane before analysis.The separation was performed on an IonPac SCS 1 analytical column（250 mm×4.0 mm i.d.） and an IonPac SCG 1 guard column（50 mm×4.0 mm i.d.） by using methanesulfonic acid（MSA） as eluent with 10% acetonitrile as organic modifier.Results：The linear range of the method was 0~200 μg/ml for ammonium and ethanolamine,both with correlation coefficients of more than 0.999,the limits of detection were 1.9 μg/L for ammonium and 2.4 μg/L for ethanolamine,respectively.The recoveries were 97.8%~101.6% for ammonium and 95.6%-99.0% for ethanolamine,both RSD less than 5.0%.Conclusion：The developed method is satisfactory for the determination of ammonium and ethanolamine in hair dye.
出处
《中国卫生检验杂志》
CAS
2010年第3期508-509,共2页
Chinese Journal of Health Laboratory Technology
