摘要
背景:盐酸小檗碱的广谱抗菌作用在口腔科主要用于治疗复发性口疮、根尖周炎、放射性口腔黏膜炎和冠周炎等,但其治疗牙周炎的作用机制尚待研究。目的:实验希望通过观察盐酸小檗碱对大鼠实验性牙周炎模型牙周组织及相关细胞因子表达的影响,揭示和认识盐酸小檗碱在口腔组织修复中的作用途径。方法:3月龄健康160～200gWistar大鼠60只,采用局部钢丝结扎结合全身肌注醋酸波尼松龙方法,成功建立大鼠实验性牙周炎模型。将造模成功的40只大鼠随机分为模型组(8只)和治疗组(32只),同时取10只正常大鼠作对照。治疗组每日每只大鼠灌服盐酸小檗碱0.06g/kg,模型组灌服等剂量生理盐水。治疗组动物分别于灌药后第1,2,3,4周末处死(每次8只),模型组和正常对照组第4周末处死。主要观察:①口腔大体观察及X射线检查。②牙周组织病理学检查。③采用免疫组织化学SABC法检测肿瘤坏死因子α、骨钙素、白细胞介素1β、白细胞介素6水平。结果与结论:①模型组大鼠在注射激素后牙龈组织糜烂溢脓;治疗组上述症状缓解;正常组牙周组织无异常。X射线观察模型组牙槽嵴吸收,根间阴影明显。②牙周炎模型组牙周组织出现明显的病理改变,其肿瘤坏死因子α、白细胞介素1β、白细胞介素6表达明显高于正常组,骨钙素表达明显低于正常组(P<0.05);治疗组的牙周组织显示炎症修复期的病理改变,其肿瘤坏死因子α、白细胞介素1β、白细胞介素6表达明显低于模型组,骨钙素明显高于模型组(P<0.05)。结果提示,盐酸小檗碱能促进大鼠实验性牙周炎模型牙周组织的修复和骨钙素的表达,同时抑制肿瘤坏死因子α、白细胞介素1β、白细胞介素6的表达。
BACKGROUND：The broad-spectrum antibacterial action of berberine hydrochloride mainly contributes to recurrent aphtha,periapical periodontitis,radioactive mucositis and pericoronitis,however a little evidence support the action mechanism underlying periodontitis treatment. OBJECTIVE：To determine the effects of berberine hydrochloride on the expressions of related cytokines in periodontal tissues of experimental periodontitis rats,to reveal and understand the action pathway of berberine hydrochloride on oral tissue repair. METHODS：Sixty Wistar rats weighing 160-200 g,aged 3 months Models,were involved in this study. Models of experimental periodontitis were established in rats through a use of local steel-wire ligation and systemic injection of prednisone acetate. Forty successfully established models were randomized into periodontitis model group （n=8） and periodontitis treatment group （n=32）,at the same time,10 normal rats served as control group. The treatment group of animals were fed with 0.06 g/kg berberine hydrochloride daily and medicated to death over the 1,2,3,4 weekends （8 rats each）. The model group was fed with isodose normal saline. The model group and normal control group were killed at the fourth weekend. Main observations：①Oral gross observation and X-ray film examination; ②Pathological assay of periodontal tissues; ③Immunohistochemical SABC method was conducted to determine the expressions of tumor necrosis factor-α （TNF-α）,bone gla protein （BGP）,interleukin-1β （IL-1β）,interleukin-6 （IL-6） in periodontal tissues in rats. RESULTS AND CONCLUSION：①Following hormone injection,gum tissue exhibited erosion and pyorrhea in model group of rats; the above-mentioned symptoms were relieved in rats of treatment group; there was no abnormality in periodontal tissues of normal rats. X-ray examination revealed alveolar crest resorption and obvious interradicular shadow in the model group. ② Rats of model group showed obvious pathologic changes in periodontal tissues,the levels of TNF-α,IL-1β,IL-6 were significantly higher and the level of BGP was dramatically lower than those in normal group （P 0.05）; Treatment with berberine hydrochloride decreased the levels of TNF-α,IL-1β,IL-6 in periodontal tissues and increased the level of BGP compared with model group （P 0.05）. The periodontal tissues in groups treated with berberine hydrochloride exhibited pathological changes at inflammatory repair stage. Results showed that berberine hydrochloride inhibits the expressions of TNF-α,IL-1β,IL-6 in periodontal tissues in experiment rat models of periodontitis,and promotes the expression of BGP and repair of periodontal tissue.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第2期370-374,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
