摘要
对山西省果树研究所、北京市林业果树研究所和北京市昌平果树研究所保存的28份SH系苹果砧木进行鉴定,结果表明:山西果树所的SH9与北京林果所的SH9,相似性高达100%,属于同名同物;山西果树所的SH17、SH18、SH40与北京昌平的SH17、SH18、SH40的相似性分别为68.9%、55.1%和65.6%,属于同名异物;山西果树所、北京林果所及北京昌平的SH38,三者间最高相似性为61.2%,均为不同材料;山西的SH18与SHb(100%),北京昌平的SH17与山西的SHd,山西的SHa与山西的SHe,山西的SH18(28)与山西的SH40,相似性均在99%以上,属于同一种材料,而相似性比较高的还有昌平的SH18与昌平的SH38(98.4%),并且山西的SH12与山西的SH17及SH29三者相似性在97.2%～98.9%之间;不同保存地的SH1、SH3、SH6、SH8-11、SH9-hf、SH19、SH28、SH29、SHc、SHd和SHf为异名异物材料。
The phenomenon of homonym and synonym of SH series apple rootstocks happened in the process of introduction breeding,which was also proved in the practice of planting.Therefore,it was necessary to clarify stem and series of homonym and synonym.With 28 SH series apple rootstocks preserved in Pomo-logy Insitute of Shanxi（Shanxi）,Institute of Forestry and Pomology of Beijing （Beijing） and Institute of Pomology of Changping,Beijing （Changping,Beijing） as materials,used method of simple sequence repeat （SSR） molecular markers,screened 82 pairs primer from 112 pairs to conduct PCR amplification,analyzed results of polyacrylamide gel electrophoresis and conducted data analysis of bands data matrices by NTSYS software.The results showed that among homogeneity in different institutes,SH9 in Shanxi and Beijing has 100% similarity;There were four homonyms in the samples,i.e.the similarity coefficients of SH17,SH18,SH40 coming from different conservative places were as low as 68.9%,55.1% and 65.6% respectively and among the similarity coefficients of SH38 coming from different conservative places the highest was 61.2%.Three heteronyms were also identified：SH18 （Shanxi） and SHb（100%）,SH17 （Changping,Beijing） and SHd（99.5%）,SHa and SHe（99.5%）,SH18（28） and SH40 （Shanxi） （99%）,all of them had a similarity coefficient larger than 99%.In addition,high similarities were found between SH18 and SH38 from Changping （98.4%） and among SH12,SH17（Shanxi）and SH29 （97.2%-98.9%）.SH1,SH3,SH6,SH8-11,SH9-hf,SH19,SH28,SH29,SHc,SHd and SHf etc.in different conservative places were heterogeneous.
出处
《园艺学报》
CAS
CSCD
北大核心
2010年第2期303-306,共4页
Acta Horticulturae Sinica
基金
国家苹果产业技术体系建设专项项目(MATS)
国家高技术研究与发展计划(‘863’)项目(2008AA10Z157)
