摘要
目的观察单侧输尿管梗阻(UUO)致大鼠肾间质纤维化的血液流变学及微循环的变化特点,从络病角度探讨肾间质纤维化的中医病机。方法建立单侧输尿管梗阻致大鼠肾间质纤维化模型,观察肾间质纤维化大鼠全血粘度150/s切变率、30/s切变率、5/s切变率、1/s切变率、红细胞压积、舌下及耳廓的脉络变化,同时检测肾纤维化指标、血清肌酐及尿素氮。结果肾间质纤维化模型组的全血粘度150/s切变率、30/s切变率、5/s切变率、1/s切变率、红细胞压积、透明质酸酶(HA)、层粘连蛋白(LN)、Ⅲ型前胶原(PCⅢ)、IV型胶原(C IV)、血清肌酐(SCr)、尿素氮(BUN)与假手术组相比显著升高;且肾纤维化指标(HA、LN、PCⅢ、C IV)与全血粘度的各个切变率及红细胞压积呈显著正相关。结论肾间质纤维化大鼠血流变及微循环的变化特点一定程度上反应了肾间质纤维化"络脉瘀组"的中医病机,为抗肾间质纤维化"活血通络"的治疗提供初步依据。
Objective To observe the changes of blood rheology and microcirculation in renal interstitial fibrosis and unilateral ureteral obstruction（UUO）.Methods Rat fibrosis models was established by unilateral ureteral obstruction.Hematocrit and blood viscosity at shear rate in 150/s,30/s,5/s,1/s,renal fibrosis serum markers,serum creatinine and blood urea nitrogen were detected respectively in sham operation and model group;microcirculation microscope was used to observe the thread of hypoglottis and auricle in rats.Results The indexes of hematocrit,blood viscosity at different shear rate,hyaluronic acid（HA）,laminin（LN）,procolagen type Ⅲ（PCⅢ）,collagen typeⅣ（CⅣ）,serum creatinine（SCr） and blood urea nitrogen（BUN） were increased significantly after fibrosis appeared,and there had positive correlation between blood viscosity at different shear rate,hematocrit and serum concentration of the fibrosis markers（HA,LN,PCⅢ,C IV）.Conclusion The tendency of blood rheological and microcirculation changes hint the pathogenesis of renal interstitial fibrosis including ＂blood-stasis blocking collaterals＂.This may be one of the causes about using ＂promoting blood circulation to remove meridian obstruction＂ method to treat renal interstitial fibrosis.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2010年第3期736-738,共3页
Lishizhen Medicine and Materia Medica Research
基金
广东省广州市科技计划项目(No.2008Z1-E461-1)
