摘要
根据Gluconobacter oxydans合成2-酮基-L-古龙酸(2-KLG)代谢途径采用单因素实验研究了6种金属离子对细胞生长和2-KLG的影响,在此基础上,对促进细胞生长或产酸的Mg2+、Mn2+、Fe3+建立多元二次方程模型,得到上述3种金属元素的最佳浓度为Fe3+0.21 mmol/L,Mn2+9.98 mmol/L和Mg2+4.23 mmol/L。在这一最优组合下,2-KLG产量达到65.1 g/L,与优化前比较,提高了144.4%。
To further increase the production of 2-keto-L-gulonic acid by Gluconobacter oxydans,the effects of six different metal ions were determined,based on the analysis of L-Sorbosone pathway.It was found that Fe3＋,Mg2＋,Mn2＋ can enhance the cell growth and 2-KLG production.Then,an optimum concentration of those three metal ions was achieved by developing a multivariate quadratic equation： Fe3＋ 0.21 mmol/L,Mn2＋ 9.98 mmol/L,and Mg2＋ 4.23 mmol/L.By using the optimum combination,the production of 2-keto-L-gulonic reached at 65.1 g/L,which is 144.4% higher than that of the control.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2010年第1期139-144,共6页
Journal of Food Science and Biotechnology
基金
国家863计划项目(2006AA020303)
国家“十一五”科技支撑计划项目(2007BAI46B02)
