摘要
动物α干扰素具有高效广谱的抗病毒作用,通过农杆菌介导法将35S驱动的干扰素基因ChIFN-α转化烟草,Southern杂交和W estern杂交进行目的基因的转化、表达检测,重组蛋白的数量通过ELISA测定,对获得的转基因烟草植株进行TMV接种实验,结果表明ChIFN-α基因的转化赋予了转基因烟草对TMV的抗性,进一步的荧光定量PCR差异表达检测结果表明,该抗性可能与转基因烟草体内防卫基因PR-1 a、抗病N基因和信号转导MAPK基因受到上调表达有关。
Alpha interferon is a pleiotropic animal cytokine that possess powerful and wide-range of antiviral properties.The expression vector carrying chicken alpha interferon gene（ChIFN-α） driven by 35S promoter has been introduced into Nicotiana tabacum L.Xanthin by Agrobacterium transformation in our work.The transgenic plants were detected by Southern blot and Western blot.Recombinant ChIFN-α protein in tobacco leaf was analyzed by enzyme-linked immunosorbent assay（ELISA）.The results of tobacco mosaic virus（TMV） challenge test showed that transgenic tobacco possessed certain resistance to TMV infection.And this might be related to the up-regulated expression of PR-1a、N and MAPK genes in transgenic plants after TMV infection.
出处
《中国农业科技导报》
CAS
CSCD
2010年第1期118-122,共5页
Journal of Agricultural Science and Technology
基金
"十一五"国家科技支撑计划项目(2007BAD59B06)
国际科技合作资助项目(2007DFA31260)
国家转基因新品种培育重大专项(2008ZX08010-003)资助
关键词
鸡α干扰素
转基因烟草
烟草花叶病毒
抗病性
chicken alpha interferon
transgenic tobacco
tobacco mosaic virus
virus resistance
