摘要
【目的】研究贡嘎蝠蛾幼虫肠道优势菌肉杆菌(Carnobacterium sp.)Hg4-03作为食物添加剂对实验室饲养的4龄贡嘎蝠蛾幼虫肠道菌群的影响。【方法】采用16S rDNA序列与PCR/DGGE(Denaturing gradient gel electrophoresis)分析技术相结合的方法,健康幼虫被随机分为处理组1、处理组2和对照组,两组处理组分别饲喂添加不同浓度肉杆菌Hg4-03的天然饲料,对照组只饲喂天然饲料。14 d和28 d后每组随机解剖6条幼虫,收集肠道样品,经细菌通用引物扩增细菌16S rDNA,DGGE分离并进行细菌多样性图谱分析。【结果】饲喂肉杆菌Hg4-03后幼虫肠道菌群的多样性指数呈上升趋势;处理组幼虫肠道中肉杆菌Hg4-03含量增加,且处理组中枯草芽孢杆菌(Bacillus subtilis)的量也明显增加。【结论】将肉杆菌Hg4-03作为益生菌饲喂贡嘎蝠蛾幼虫有助于维持幼虫肠道菌群多样性平衡,这为贡嘎蝠蛾人工或半人工养殖提供了一定的参考价值。
[ Objective] We investigated the influence of Carnobacterium sp. Hg4-03 on intestinal microflora of the 4th instar Hepialus gonggaensis larvae, an economically important insect. [ Methods] The effect was observed by molecular techniques based on the sequence of 16S rDNA V3 region gene and PCR/DGGE （Denaturing gradient gel electrophoresis）. The health larvae were divided randomly into treatment groupl, treatment group2 and control group, and fed on their natural food added with different amount of Carnobacterium sp. Hg4-03 （ group 1 and group 2） or ddH2O （control）. After 14 days and 28 days, 6 larvae from each group were randomly selected to be dissected and contents of guts were collected for bacterial diversity analysis. [ Results] The result showed that bacterial diversity index of treatment groups with Hg4-03 was higher than control group, and the species of bacteria increased. Comparing the bands of DGGE profiles between three groups, the intensity of band stand for Carnobacterium sp. Hg4-03 have enhanced. Another band with swelled intensity found in profiles come from treatment group2 after 28 d fed, the sequence analysis showed it belongs to Bacillus subtilis. [ Conclusion] Feeding with Carnobacterium sp. Hg4-03 could balance the bacteria in larvae gut and increase bacteria diversity, that means the bacteria made a positive effect on the bacterial diversity of H. gonggaensis larval gut. Hg4-03 may be used as probiotic bacteria for rearing H. gonggaensis larvae in controlling conditions or the larvae＇ s hemi-wild rearing.
出处
《微生物学报》
CAS
CSCD
北大核心
2010年第2期251-255,共5页
Acta Microbiologica Sinica
基金
国家自然科学基金(30572325)~~
